M0446

Minimum Essential Medium Eagle, with Earl's salts

Name: Minimum Essential Medium Eagle, with Earl's salts
Brand: SIGMA
Price: 32.9 EUR

StableCell^™, with Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

MEM

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500 ML

€ 32,90

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Über diesen Artikel

UNSPSC Code:

12352207

NACRES:

NA.75

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Produktname

Minimum Essential Medium Eagle, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO₂): no
NaHCO₃: yes
Earle’s salts (5% CO₂): yes
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Dieser Artikel	M4655	FG0325	51416C
Sigma-Aldrich M0446 StableCell^™ MEM	Sigma-Aldrich M4655 Minimum Essential Medium Eagle	SAFC FG0325 Minimum Essential Medium Eagle	SAFC 51416C Minimum Essential Medium
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form liquid
components sodium pyruvate: no HEPES: no Hanks’ salts (2% CO₂): no NaHCO₃: yes Earle’s salts (5% CO₂): no stable glutamine: yes phenol red: yes L-glutamine: no glucose: yes	components sodium pyruvate: no phenol red: yes Earle’s salts (5% CO₂): yes HEPES: no NaHCO₃: yes L-glutamine: yes	components stable glutamine: yes L-glutamine: no phenol red: yes sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no Hanks’ salts (2% CO₂): no glucose: yes NaHCO₃: yes	components Earle’s salts (5% CO₂): yes L-glutamine: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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