I have been struggled with high background using other companies Ulex, but this one just worked perfectly since first time.
Fortfahren mit
L8146
Lektin aus Ulex europaeus (Ginster, Stechginster) aus Ulex europaeus (gorse, furze)
peroxidase conjugate, lyophilized powder
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
-NotTranslated-
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
0.5 mg | Warenkorb auf Verfügbarkeit prüfen | € 311,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
70.00-90.00% (Lowry)
Biological source:
Ulex europaeus
€ 311,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
Ulex europaeus
Quality Segment
conjugate
peroxidase conjugate
assay
70.00-90.00% (Lowry)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
composition
Protein, ~80% Lowry
technique(s)
western blot: suitable
storage temp.
2-8°C
Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Packaging
Package size based on protein content.
Physical form
Contains sodium citrate buffer salts
Preparation Note
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Other Notes
Conjugates are prepared from affinity purified lectin.
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description peroxidase conjugate, lyophilized powder | description biotin conjugate, lyophilized powder | description peroxidase conjugate, lyophilized powder | description Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture |
| assay 70.00-90.00% (Lowry) | assay - | assay - | assay - |
| biological source Ulex europaeus | biological source - | biological source Arachis hypogaea (peanut) | biological source - |
| technique(s) western blot: suitable | technique(s) - | technique(s) western blot: suitable | technique(s) cell culture | mammalian: suitable |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| potency <40 μg/mL agglutination activity | potency <50 μg/mL agglutination activity | potency - | potency ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells), erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.) |
Still not finding the right product?
Explore all of our products under Lektin aus Ulex europaeus (Ginster, Stechginster) aus Ulex europaeus (gorse, furze)
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.