Yes, this product is suitable for use in Neuraminidase Inhibition assays.
Fortfahren mit
L7759
Lektin aus Arachis hypogaea (Erdnuss)
peroxidase conjugate, lyophilized powder
Synonym(e):
Erdnuss-Agglutinin, PNA
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
250 μg | Warenkorb auf Verfügbarkeit prüfen | € 132,00 |
1 mg | Warenkorb auf Verfügbarkeit prüfen | € 427,00 |
Über diesen Artikel
NACRES:
NA.54
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Arachis hypogaea (peanut)
Technischer Dienst
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Unterstützung erhaltenbiological source
Arachis hypogaea (peanut)
Quality Segment
conjugate
peroxidase conjugate
form
lyophilized powder
peroxidase activity
≥20 units/mg protein
technique(s)
western blot: suitable
storage temp.
−20°C
Application
Allgemeines Western Blot-Protokoll:
- Glykoproteinprobengröße: 500 ng
- Lektinkonzentration: 0,1 ug/ml
- Die Proben mit 500 ng Glykoprotein pro Spur laden
- Mit 4 - 20 % Bis-Tris SDS-PAGE-Gel ausführen
- Das Gel auf eine PVDF-Membran transferieren
- Die Membrane 1 Stunde bei RT mit RIPA-Puffer (R0278 Sigma) blocken
- HRP-Lektin zu 0,1ug/ml mit RIPA-Puffer für 2 Stunden bei RT inkubieren
- Die Membran 5 x 5 Minuten lang mit 25 ml RIPA-Puffer waschen
- Mittels chemilumineszentem Substrat (CPS1-120) detektieren
Biochem/physiol Actions
PNA verklumpt keine normalen humanen Erythrozyten, bewirkt aber eine starke Verklumpung (Agglutination) von mit Neuraminidase behandelten Erythrozyten. PNA weist eine potente Anti-T-Aktivität auf, ähnlich der Anti-T-Aktivität in Humanseren. Das Lektin kann für die Unterscheidung zwischen humanen Lymphozyten-Untergruppen verwendet werden.
Physical form
Enthält Natriumcitrat
Preparation Note
Other Notes
Eine Einheit bildet 1 mg Purpurogallin in 20 s ab Pyrogallol bei pH 6,0 bei 20 °C.
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Dieser Artikel | |||
|---|---|---|---|
| description peroxidase conjugate, lyophilized powder | description lyophilized powder, Affinity-purified | description FITC conjugate, lyophilized powder | description Type II, lyophilized powder, ≥60 units/mg protein |
| biological source Arachis hypogaea (peanut) | biological source - | biological source - | biological source Arachis hypogaea |
| technique(s) western blot: suitable | technique(s) - | technique(s) - | technique(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate peroxidase conjugate | conjugate - | conjugate FITC conjugate | conjugate - |
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Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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Can I use it for NI assay? Previously I used #L6135-1MG.
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein? If so, what is the carbohydrate content?
1 answer-
Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate. Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure". Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).
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