L2898

Lysostaphin aus Staphylococcus staphylolyticus

aseptically filled

• Synonym(e): Glycyl-glycine Endopeptidase
• CAS-Nummer: 9011-93-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• Specific activity: >500 units/mg protein

Eigenschaften

• sterility: aseptically filled
• Quality Segment: 200
• form: powder
• specific activity: >500 units/mg protein
• mol wt: 25 kDa
• composition: Protein, 40-70% biuret
• input: bacteria and yeast sample(s)
• antibiotic activity spectrum: Gram-positive bacteria
• mode of action: cell wall synthesis | interferes
• storage temp.: −20°C

Beschreibung

General description

Lysostaphin is mainly liberated as a proenzyme. It is highly stable at pH 4 and temperature 5°C.

Chemical structure: peptide

Biochem/physiol Actions

Lysostaphin exhibit a lytic action against Staphylococcus aureus. It possesses several functions of three major enzymes, such as, glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin can be used to treat antibiotic-resistant staphylococcal infections. Lysostaphin being an antistaphylococcal agent, can be used as a preservative in the food industry and in clinical labs for rapid screening.

Lysostaphin ist eine Zink-Endopeptidase mit einer Molekülmasse von etwa 25 kDa. Da Lysostaphin die Polyglycinvernetzungen in der Peptidoglykanschicht der Zellwand von Staphylococcus-Spezies spaltet, ist es nützlich für die Zelllyse und auch als potenzielles antimikrobielles Therapeutikum.
Optimaler pH-Wert für Aktivität: ~7,5

Preparation Note

Prepared from L 7386

Other Notes

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)