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L2898

Lysostaphin aus Staphylococcus staphylolyticus

aseptically filled

Synonym(e):

Glycyl-glycine Endopeptidase

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Über diesen Artikel

CAS-Nummer:
9011-93-2
UNSPSC Code:
12352204
NACRES:
NA.54
EG-Nummer:
3.4.24.75 (BRENDA, IUBMB)
MDL number:
MFCD00131556
Specific activity:
>500 units/mg protein

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Unterstützung erhalten

sterility

aseptically filled

form

powder

specific activity

>500 units/mg protein

mol wt

25 kDa

composition

Protein, 40-70% biuret

antibiotic activity spectrum

Gram-positive bacteria

mode of action

cell wall synthesis | interferes

storage temp.

−20°C

Quality Level

200

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Dieser Artikel
SRE0053L7386SAE0091
Lysostaphin from Staphylococcus staphylolyticus aseptically filled

Sigma-Aldrich

L2898

Lysostaphin from Staphylococcus staphylolyticus

Lysostaphin from Staphylococcus staphylolyticus Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

Sigma-Aldrich

SRE0053

Lysostaphin from Staphylococcus staphylolyticus

Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Sigma-Aldrich

L7386

Lysostaphin from Staphylococcus staphylolyticus

Lysostaphin from Staphylococcus staphylolyticus free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

Sigma-Aldrich

SAE0091

Lysostaphin from Staphylococcus staphylolyticus

specific activity

>500 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥500 units/mg protein

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

25 kDa

mol wt

26,926 Da

mol wt

25 kDa

mol wt

27 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

sterility

aseptically filled

sterility

-

sterility

-

sterility

-

Quality Level

200

Quality Level

400

Quality Level

300

Quality Level

-

General description

Lysostaphin is mainly liberated as a proenzyme. It is highly stable at pH 4 and temperature 5°C.
Chemical structure: peptide

Biochem/physiol Actions

Lysostaphin exhibit a lytic action against Staphylococcus aureus. It possesses several functions of three major enzymes, such as, glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin can be used to treat antibiotic-resistant staphylococcal infections. Lysostaphin being an antistaphylococcal agent, can be used as a preservative in the food industry and in clinical labs for rapid screening.
Lysostaphin ist eine Zink-Endopeptidase mit einer Molekülmasse von etwa 25 kDa. Da Lysostaphin die Polyglycinvernetzungen in der Peptidoglykanschicht der Zellwand von Staphylococcus-Spezies spaltet, ist es nützlich für die Zelllyse und auch als potenzielles antimikrobielles Therapeutikum.
Optimaler pH-Wert für Aktivität: ~7,5

Preparation Note

Prepared from L 7386

Other Notes

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000493980
0000493980
SLCR3968
SLCR3968
SLCG3890

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)
Igor Belyansky et al.
The Journal of surgical research, 171(2), 409-415 (2011-06-24)
Lysostaphin (LS), a naturally occurring Staphylococcal endopeptidase, has the ability to penetrate biofilm, and has been identified as a potential antimicrobial to prevent mesh infection. The goals of this study were to determine if LS adhered to porcine mesh (PM)
Fuying Cui et al.
Drug delivery, 17(8), 617-623 (2010-08-24)
Nasal colonization of Staphylococcus aureus (S.aureus) is known as a significant risk factor for nosocomial infections, and clearance of its nasal colonization greatly reduces the risk. In the present study the preparation and characterizations of the chitosan-o/w cream incorporated with
M Fenton et al.
Journal of applied microbiology, 111(4), 1025-1035 (2011-08-05)
To develop an efficient purification strategy for the bacteriophage lysin CHAP(K) . To evaluate its antibacterial spectrum(,) enzymatic properties, optimal reaction conditions and lytic activity against live Staphlyococcus aureus. Recombinant CHAP(K) was purified to homogeneity by cation exchange chromatography, with
A P Desbois et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 30(8), 1015-1021 (2011-02-12)
Drug-resistant staphylococci constitute a serious problem that urgently requires the discovery of new therapeutic agents. There has been a resurgence in interest in using lysostaphin (a specific anti-staphylococcal enzyme) as a treatment for infections caused by these important pathogens. However
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Questions

  1. Which reaction buffer is recommended for this enzyme? Are there any limitations, inhibitors, etc? Would it work in any medium.

    1 answer

    1. Please see the link below to the quality control test procedure utilized for the enzymatic assay of lysostaphin. Reaction with other buffers has not been tested and, please refer to academic literature for guidance on how other buffers may influence the enzyme's reactivity:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/120/423/l7386enz.pdf

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