I8657

Interferon α from rat

≥95%, recombinant, expressed in bacteria, lyophilized powder, suitable for cell culture

• Synonym(e): IFN-α
• MDL-Nummer: MFCD04040884
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 200
• Rekombinant: expressed in bacteria
• Assay: ≥95%
• Form: lyophilized powder
• Mol-Gew.: ~19 kDa
• Verpackung: pkg of 1 vial
• Konzentration: 300,000-800,000,000 units/mL
• Methode(n): cell culture | mammalian: suitable
• UniProt-Hinterlegungsnummer: P05011
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: rat ... Ifna_mapped(24480)

Beschreibung

Allgemeine Beschreibung

Interferon α is a cytokine, that belongs to type I interferon (IFNs) family.[1] It possesses 14 genes, including 2 pseudo-genes or allelic forms that encodes for 13 proteins.[2] It is mainly induced in response to viral infection.[1] It is a non-leukemogenic peptide.[2]

Biochem./physiol. Wirkung

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes) and ruminant embryos (τ subtypes). Interferon induces a variety of effects on target cells including antiviral, antiproliferative and immunomodulatory activities.

Interferon α play a key role in the inhibition of in vitro proliferation of hematopoietic progenitors.[2] It mediates the regulation of gene expression that is responsible for development and functionality of innate and adaptive immunity.[3] It has been used as myelosuppressive agent for myeloproliferative neoplasms (MPNs).[2]

Physikalische Form

Lyophilized from a solution containing citric acid buffered saline (CBS pH 5.0) and 125 mM trehalose

Hinweis zur Analyse

The biological activity is determined in the cytopathic effect inhibition assay using rat epithelial-like (RATEC) cells with vesicular stomatitis virus (VSV) in the presence of 2mM glutathione. The cytopathic effect of 50% is produced with ~1 unit/mL interferon. Since there is no international rat interferon α standard, units are determined with respect to the NIH international standard reference for mouse interferon α/β.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable