I7385

Monoklonaler Anti-Human IgG4

clone HP-6025, purified from hybridoma cell culture

• Synonym(e): Anti-Human IgG4 antibody, Mouse monoclonal
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: HP-6025, monoclonal
• Application: ELISA (i), IF
• Citations: 14

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: HP-6025, monoclonal
• form: (Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide)
• contains: 15 mM sodium azide
• technique(s): immunofluorescence: suitable, indirect ELISA: 1:5,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines. Unlike other immunoglobulin G is rare and constitutes to only 3-4 percent of the total IgG in the serum.

Application

In der IUIS/WHO2 Studie wurde dieser monoklonale Antikörper als einer der am vielseitigsten einsetzbare IgG4-spezifische monoklonale Antikörper hervorgehoben. Monoklonaler Anti-Human-IgG4 kann für die Identifizierung der IgG4-Unterklasse durch verschiedene Immunoassays verwendet werden, darunter: ELISA, Imprint-Immunfixation (IIF), Immunfluorometrischer Assay (IFMA), Hämagglutination (HA), Hämagglutination-Hemmung (HAI), Particle Counting Immunoassay (PACIA) und Detektion von cytoplasmischen IgG.

Monoclonal Anti-HuIgG4 man antibody produced in mouse has been used in ELISA and immunofluorescence.

Biochem/physiol Actions

Monoklonaler Anti-Human IgG4 ist spezifisch für die IgG4-Unterklasse und nicht reaktiv mit IgG1, IgG2 und IgG3 im ELISA.

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differing from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4. Increased levels of IgG4 is observed in atopic dermatitis, parasitic diseases, pemphigus vulgaris and pemphigus foliaceus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable