B3648

Monoklonaler Anti-Human IgG4-Biotin

clone HP-6025, purified from hybridoma cell culture

• Synonym(e): Monoklonaler Anti-Human IgG4
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: biotin conjugate
• Clone: HP-6025, monoclonal
• Application: ELISA (d)
• Citations: 35

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: HP-6025, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): direct ELISA: 1:60,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

Application

In der IUIS/WHO2 Studie wurde dieser monoklonale Antikörper als einer der am vielseitigsten einsetzbare IgG4-spezifische monoklonale Antikörper hervorgehoben. Monoklonaler Anti-Human-IgG4 kann für die Identifizierung der IgG4-Unterklasse durch verschiedene Immunoassays verwendet werden, darunter: ELISA, Imprint-Immunfixation (IIF), Immunfluorometrischer Assay (IFMA), Hämagglutination (HA), Hämagglutination-Hemmung (HAI), Particle Counting Immunoassay (PACIA) und Detektion von cytoplasmischen IgG.

Anti-Human IgG4−Biotin antibody, Mouse monoclonal has been used in immunohistochemistry and immunostaining.

Monoclonal Anti-Human IgG4-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:15,000. It was used for detection of serum IgG4 produced in response to malaria parasite infection by ELISA.

Biochem/physiol Actions

Monoklonaler Anti-Human IgG4 ist spezifisch für die IgG4-Unterklasse und nicht reaktiv mit IgG1, IgG2 und IgG3 im ELISA.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG4 antibody allows for the binding of various labels such as avidin or streptavidin.

IgG4 is the least abundant immunoglobulin present in the human serum. IgG4 is implicated in a unique process called fab-arm exchange (FAE) and limits the formation of immune complex. It has anti-inflammatory properties. IgG4 functions as a therapeutic antibody. It provides protection against allergy, prevents mast cell degranulation and has a harmful role in malignant melanoma. IgG4 may be associated with autoimmune diseases such as, autoimmune pancreatitis (AIP) type 1 and 2, Mikulicz syndrome, Kuttner′s tumor, Riedel′s thyroiditis and tubulointerstitial nephritis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Other Notes

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Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable