Unforunately we do not have information on the cross-reactivity of the antibody B3648-.5ml with the cynomolgus. Additionally, no in-house epitope mapping has been conducted on this antibody. However, Robert G. Hamilton and Sherie L. Morrison, in their 1993 article in the Journal of Immunological Methods, have performed some further characterization. According to the article, "The anti-human IgG4 MAbs (HP6023, HP6025) bound to a similar epitope on the carboxyl terminus of CH2 or the CH3 of human IgG4." For more details, please refer to the article: "Journal of Immunological Methods Volume 158, Issue 1, 14, Pages 107-122."
B3648
Monoklonaler Anti-Human IgG4-Biotin
clone HP-6025, purified from hybridoma cell culture
Synonym(e):
Monoklonaler Anti-Human IgG4
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0.2 ML
€ 407,00
0.5 ML
€ 810,00
Über diesen Artikel
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
HP-6025, monoclonal
Application:
ELISA (d)
Citations:
35
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Unterstützung erhaltenbiological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
HP-6025, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:60,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| conjugate biotin conjugate | conjugate biotin conjugate | conjugate biotin conjugate | conjugate biotin conjugate |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| technique(s) direct ELISA: 1:60,000 | technique(s) direct ELISA: 1:80,000 | technique(s) direct ELISA: 1:40,000 | technique(s) direct ELISA: 1:5,000 |
| species reactivity human | species reactivity rabbit, sheep, horse (IgG), goat, human | species reactivity human | species reactivity sheep, goat, bovine |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| isotype IgG1 | isotype IgG2a | isotype IgG1 | isotype IgG1 |
Application
In der IUIS/WHO2 Studie wurde dieser monoklonale Antikörper als einer der am vielseitigsten einsetzbare IgG4-spezifische monoklonale Antikörper hervorgehoben. Monoklonaler Anti-Human-IgG4 kann für die Identifizierung der IgG4-Unterklasse durch verschiedene Immunoassays verwendet werden, darunter: ELISA, Imprint-Immunfixation (IIF), Immunfluorometrischer Assay (IFMA), Hämagglutination (HA), Hämagglutination-Hemmung (HAI), Particle Counting Immunoassay (PACIA) und Detektion von cytoplasmischen IgG.
Anti-Human IgG4−Biotin antibody, Mouse monoclonal has been used in immunohistochemistry and immunostaining.
Monoclonal Anti-Human IgG4-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:15,000. It was used for detection of serum IgG4 produced in response to malaria parasite infection by ELISA.
Biochem/physiol Actions
Monoklonaler Anti-Human IgG4 ist spezifisch für die IgG4-Unterklasse und nicht reaktiv mit IgG1, IgG2 und IgG3 im ELISA.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG4 antibody allows for the binding of various labels such as avidin or streptavidin.
IgG4 is the least abundant immunoglobulin present in the human serum. IgG4 is implicated in a unique process called fab-arm exchange (FAE) and limits the formation of immune complex. It has anti-inflammatory properties. IgG4 functions as a therapeutic antibody. It provides protection against allergy, prevents mast cell degranulation and has a harmful role in malignant melanoma. IgG4 may be associated with autoimmune diseases such as, autoimmune pancreatitis (AIP) type 1 and 2, Mikulicz syndrome, Kuttner′s tumor, Riedel′s thyroiditis and tubulointerstitial nephritis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklasse
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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IgG4-related autoimmune diseases: Polymorphous presentation complicates diagnosis and treatment
Kleger A, et al.
Deutsches Arzteblatt International, 112(8), 128-128 (2015)
Amre Nasr et al.
BMC immunology, 14, 38-38 (2013-08-15)
C-reactive protein (CRP) is a nonspecific, acute-phase protein that rises in response to infectious and non-infectious inflammatory processes. Infections are the single largest cause of neonatal deaths globally.The primary aim of this study is to examine the association between CRP
Human IgG4: a structural perspective
Davies AM and Sutton BJ
Immunological Reviews, 268(1), 139-159 (2015)
Intranasal air sampling in homes: relationships among reservoir allergen concentrations and asthma severity
Gore RB, et al.
The Journal of Allergy and Clinical Immunology, 117(3), 649-655 (2006)
Association of immunoglobulin G4 and free light chain with idiopathic pleural effusion
Murata Y, et al.
Clinical and Experimental Immunology, 190(1), 133-142 (2017)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B3648-.2ML | 04061833428306 |
| B3648-.5ML | 04061837514845 |
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Does the antibody B3648-.5ml cross-react with the cynomolgus monkey?
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