H4641

Ausgeglichene Salzlösung nach Hanks 10x

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

• Synonym(e): Hanks-Salze -Lösung
• NACRES: NA.75
• UNSPSC Code: 12352207

Eigenschaften

• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• components: phenol red: yes
• shipped in: ambient
• Quality Level: 400
• storage temp.: room temp

Beschreibung

General description

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salt Solution 10x has been used:

• as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium[1]
• to wash the biopsy specimens of lamina propria lymphocytes (LPLs)[2]
• as a wash solution to wash the fish cell line during removal from growth medium[3]

Preparation Note

Supplement with 0.35 g/L sodium bicarbonate at 1X.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable