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Merck

GE17-1196-01

Sigma-Aldrich

HiPrep 26/60 Sephacryl® S-300 HR

Cytiva 17-1196-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

Synonym(e):

HiPrep Chromatography Column, Sephacryl S-300 HR

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About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

Materialien

polypropylene (PP)

Mol-Gew.

10000-1500000 Da (globular proteins)

Verpackung

pkg of 1 ea

Hersteller/Markenname

Cytiva 17-1196-01

Parameter

<13 mL sample volume
<2.6 mL/min flow rate
0.15 Mpa (22 psi)

Bettvolumen

320 mL

Säule L × ID

60 cm × 26 mm

Matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

Aktive Matrixgruppe

dextran-acrylamide phase

Durchschnittliche Teilgröße

50 μm

cleaning

2-13

working range

3-11

Trenntechnik

size exclusion (SEC)

Allgemeine Beschreibung

HiPrep 26/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

Leistungsmerkmale und Vorteile

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

Eignung

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
HiPrep 26/60 Sephacryl S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-300 is used for separating midsize proteins including antibodies and serum proteins.
for using preparative separation of biomolecules of up to 13 mL sample volume

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Rechtliche Hinweise

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

This page shows how to refold proteins using gel filtration and products from Cytiva.

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Verwandter Inhalt

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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