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F2456

Sigma-Aldrich

Fluorescein (freie Säure)

Dye content 95%, Powder

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About This Item

Empirische Formel (Hill-System):
C20H12O5
CAS-Nummer:
2321-07-5
Molekulargewicht:
332.31
Farbindexnummer:
45350:1
Beilstein:
94324
EG-Nummer:
219-031-8
MDL-Nummer:
MFCD00005050
UNSPSC-Code:
12171500
PubChem Substanz-ID:
24894822
NACRES:
NA.47

product name

Fluorescein (freie Säure), Dye content 95 %

Form

powder

Qualitätsniveau

200

Zusammensetzung

Dye content, 95%

Farbe

orange to red-brown

mp (Schmelzpunkt)

320 °C (lit.)

λmax

496 nm

ε (Extinktionskoeffizient)

≥12000 at 282-286 nm
≥39000 at 236-240 nm
≥70000 at 488-492 nm

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H

InChIKey

GNBHRKFJIUUOQI-UHFFFAOYSA-N

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Allgemeine Beschreibung

Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

Anwendung

Fluorescein (free acid) has been used as a staining agent in retinal imaging.
Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H319

Gefahreneinstufungen

Eye Irrit. 2

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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