F3651

Fluoreszein-5(6)-isothiocyanat

≥90% purity (HPLC), powder

• Synonym(e): FITC
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 27072-45-3
• Molekulargewicht: 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894855
• UNSPSC Code: 12171500
• EC Number: 248-207-7
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Eigenschaften

• Produktname: Fluoreszein-5(6)-isothiocyanat, ≥90% (HPLC)
• Quality Segment: 200
• assay: ≥90% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to orange
• solubility: acetone: 1 mg/mL, clear, light yellow to yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Beschreibung

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Für die Fluoreszenzmarkierung von Proteinen (z.B. Immunofluoreszenz); H Protein-Labeling für die Gelchromatographie.

Fluorescein 5(6)-isothiocyanate has been used:

• for fluorescence labeling of bacteria
• as a standard for calibration of fluorometer
• for the labeling of exosomes

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3