E9762

Endoglycosidase F1 aus Elizabethkingia miricola

recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

• Synonym(e): Endoglycosidase F1 aus Elizabethkingia meningoseptica, Endoglycosidase F1 aus Flavobacterium meningosepticum
• EC-Nummer: 3.2.1.96 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00146843
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Konjugat: (N-linked)
• Form: buffered aqueous solution
• Spezifische Aktivität: ≥16 U/mg
• Mol-Gew.: 32 kDa
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.[1]

Anwendung

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR)[2] and to glycosylate fucosylated N-glycopeptides.[3]

Biochem./physiol. Wirkung

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.[4]

Verpackung

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.

Einheitendefinition

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

Physikalische Form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)