D1145

DMEM - high glucose

Name: DMEM - high glucose
Brand: SIGMA

With sodium bicarbonate, without ʟ-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

Dulbecco’s Modified Eagle’s Medium - high glucose

Synonym(e):

DME, DMEM

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UNSPSC Code:

12352207

NACRES:

NA.71

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Produktname

Dulbecco Modifiziertes Eagle-Medium – hoher Glucosegehalt, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO₃: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Dieser Artikel	D6171	D0422	D7777
Sigma-Aldrich D1145 Dulbecco′s Modified Eagle′s Medium - high glucose	SAFC D6171 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D0422 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D7777 Dulbecco′s Modified Eagle′s Medium - high glucose
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form powder
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
components L-glutamine: no sodium pyruvate: no glucose: high phenol red: no NaHCO₃: yes HEPES: no	components sodium pyruvate: no HEPES: 25 mM glucose: high NaHCO₃: yes L-glutamine: no phenol red: yes	components glucose: high phenol red: yes L-glutamine: no sodium pyruvate: yes	components glucose: high sodium pyruvate: yes NaHCO₃: no HEPES: no phenol red: yes L-glutamine: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) ist eine Modifikation des Basal Medium Eagle (BME) mit vierfacher Konzentration der Aminosäuren und Vitamine. Die ursprüngliche Formulierung enthielt 1000 mg/l Glucose und diente zur Kultivierung von embryonalen Mäusezellen. Seitdem wurde es auf verschiedene Arten modifiziert, um damit Primärkulturen von Mäuse- und Hühnerzellen sowie verschiedene normale und transformierte Zellen kultivieren zu können. Jedes dieser Medien enthält eine andere Kombination von L-Glutamin und Natriumpyruvat. Außerdem wurde die Glucosekonzentration auf 4500 mg/l erhöht, dieses Medium wird als „DMEM/High“ bezeichnet.

Preparation Note

Ergänzung mit 0,584 g/l L-Glutamin.

used together

Produkt-Nr.

Beschreibung

Preisangaben

P5280

Sodium pyruvate, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

G7513

L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

P3532

Phenol Red, powder, BioReagent, suitable for cell culture

F2442

Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

S2GPU05RE

Millipore^® Stericup^® Quick Release Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea

S2GPU11RE

Millipore^® Stericup^® Quick Release Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, pack of 12 ea

SEGPU0538

Stericup^® E-GP Funnel-less Sterile Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, 38 mm (1 1/2 in.) thread, pack of 12 ea

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

2 years ago

Can this media be used to culture COS-7 cell?

1 answer
1. Technical Support
  
  2 years ago
  
  Yes, Product D1145 has been cited by the following publication to culture COS-7 cells.
  Nat Commun. 2021 Jul 5;12(1):4132.
  A synthetic circuit for buffering gene dosage variation between individual mammalian cells
  https://pubmed.ncbi.nlm.nih.gov/34226556/
  
  Helpful?

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Charlottesville VA
Review 1

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5 out of 5 stars.

Anonymous

4 years ago

Suitability of medium for immunofluorecence applic

Convenient formulation that allows cells grown to undergo processing for immunofluorescence imaging.

Recommends this product

Yes

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