LB83

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood
• Verpackung: tube of 5 μg 98062328-DNA-5UG; pkg of vial of cells 98062328-1VL
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human lymphocytes, Xeroderma pigmentosum, EBV-transformed

Beschreibung der Zelllinie

Established by EBV transformation of mononuclear cells from the peripheral blood of a 15 year old female. The patient has been diagnosed with Xeroderma pigmentosum (XP) complementation group G, showed no skin cancer or neurologic abnormalities and reduced NK activity. XP is a relatively rare recessively inherited disease with DNA repair deficiency. Fibroblasts from the same individual have been designated XP125LO.

Anwendung

DNA repair defect studies, hprt mutation analysis

Nährmedium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subkultur-Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Sonstige Hinweise

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