CHH-1

92110412, fish cardiovascular , Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• product name: CHH-1, 92110412
• Biologische Quelle: fish cardiovascular
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Fish Chum heart

Beschreibung der Zelllinie

Established from normal heart tissue from a juvenile Onchorhynchus keta. Cells are susceptible to a wide range of fish viruses.

Anwendung

Aquatic viral studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 medi

Sonstige Hinweise

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