T98G Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090213, human brain, Not specified

• Synonym(e): T 98 G Cells, T-98G Cells, T98-G Cells
• UNSPSC Code: 41106514
• Biological source: human brain
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Hyperpentaploid, modal no. 128-132
• Morphology: Not specified
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human brain
• description: Human Caucasian glioblastoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 92090213-DNA-5UG, pkg of vial of cells 92090213-1VL
• growth mode: Adherent
• karyotype: Hyperpentaploid, modal no. 128-132
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Studies on the mechanism for cessation of proliferation in quiescent cells and cells synchronised in G1 phase.

T98G has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study the efficiency of empty spiracles homeobox 2 (EMX2) in suppressing glioblastoma.

Biochem/physiol Actions

Derived from glioblastoma multiform tumour from a 61-year-old Caucasian male. Indefinite lifespan and anchorage independent but can enter viable G1 arrested state when deprived of serum.

Human Caucasian glioblastoma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 13
D5S818: 10,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 17,20

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells may become anchorage-independent if deprived of serum.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.