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Merck

B5161

Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt

≥90% (TLC), powder

Synonym(e):

Sulfosuccinimidyl biotin

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10 MG

€ 92,50

50 MG

€ 313,00

€ 92,50


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Über diesen Artikel

Empirische Formel (Hill-System):
C14H18N3NaO8S2
CAS-Nummer:
Molekulargewicht:
443.43
NACRES:
NA.46
PubChem Substance ID:
UNSPSC Code:
12352203
MDL number:
Assay:
≥90% (TLC)
Form:
powder

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Quality Level

assay

≥90% (TLC)

form

powder

solubility

H2O: 10 mg/mL, DMF: 50 mg/mL

storage temp.

−20°C

SMILES string

[Na].OS(=O)(=O)C1CC(=O)N(OC(=O)CCCCC2SCC3NC(=O)NC23)C1=O

InChI

1S/C14H19N3O8S2.Na.H/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);;

InChI key

NKHCQKKDLWGBRK-UHFFFAOYSA-N

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Dieser Artikel
B1022B4531B3640
assay

≥90% (TLC)

assay

≥90% (TLC)

assay

>98%

assay

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

H2O: 10 mg/mL, DMF: 50 mg/mL

solubility

H2O: 10 mg/mL, DMF: 50 mg/mL

solubility

DMF: 50 mg/mL

solubility

-

form

powder

form

powder

form

powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Application

Water soluble biotinylation reagent. Typically coupled to primary amine in the pH range 6.5-8.5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazardExclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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Wei-Bor Tsai et al.
Biotechnology and bioengineering, 98(2), 498-507 (2007-03-28)
Cell adhesion to a scaffold is a prerequisite for tissue engineering. Many studies have been focused on enhancing cell adhesion to synthetic materials that are used for scaffold fabrication. Previously, we showed that immobilization of biotin molecules to chondrocyte surfaces
P Pantano et al.
Analytical chemistry, 65(5), 623-630 (1993-03-01)
Dehydrogenase enzymes are immobilized onto carbon-fiber microelectrode surfaces via avidin-biotin technology and a covalently linked hydrophilic tether. The avidin-biotin coupling strategy allows the selectivity of the electrochemical measurement to be easily changed without reoptimizing the immobilization conditions. Optimized derivatization conditions
Wei-Bor Tsai et al.
Biomaterials, 26(16), 3141-3151 (2004-12-18)
Cell adhesion to synthetic biomaterials is a prerequisite for anchorage cell culture and tissue engineering. The current study investigated utilization of an avidin-biotin binding system in enhancing chondrocyte adhesion to tissue culture polystyrene (TCPS). Biotinylated chondrocytes adhered to avidin-coated TCPS
Dominic Kamps et al.
Cell reports, 33(9), 108467-108467 (2020-12-03)
Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system

Global Trade Item Number

SKUGTIN
B5161-50MG04061833258682
B5161-10MG04061833433614

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