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B0561

Sigma-Aldrich

Monoclonal Anti-BUB1 antibody produced in mouse

2 mg/mL, clone 14H5, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-Budding Uninhibited By Benzimidazoles 1

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About This Item

MDL-Nummer:
MFCD04040371
UNSPSC-Code:
12352203
NACRES:
NA.44

Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

14H5, monoclonal

Form

buffered aqueous solution

Speziesreaktivität

human

Konzentration

2 mg/mL

Methode(n)

immunocytochemistry: 4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: suitable

Isotyp

IgG1

UniProt-Hinterlegungsnummer

O43683

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... BUB1(699)

Allgemeine Beschreibung

BUB1 is spindle checkpoint kinase that modulates kinetochore-independent segregation of chromosomes and cell proliferation. This kinase regulates the phosphorylation of MAD1 and itself. As a result, BUB1 mediates chromosome-spindle attachments during mitosis. BUB1 also phosphorylates Cdc20 which subsequently facilitates accurate checkpoint signaling . Monoclonal Anti-BUB1 antibody is specific for human BUB1 (approx. 150 kDa).

Immunogen

recombinant human BUB1.

Anwendung

Monoclonal Anti-BUB1 antibody is suitable for use in western blot, indirect ELISA, microarray, immunocytochemistry (4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol) and immunoprecipitation.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Zhanyun Tang et al.
Molecular cell, 16(3), 387-397 (2004-11-05)
To ensure the fidelity of chromosome segregation, the spindle checkpoint blocks the ubiquitin ligase activity of APC/C(Cdc20) in response to a single chromatid not properly attached to the mitotic spindle. Here we show that HeLa cells depleted for Bub1 by
B Ouyang et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 9(10), 877-885 (1998-10-28)
Eukaryotic cells have evolved a mechanism that delays the onset of anaphase until chromosomes are properly positioned on the spindle. To understand the molecular basis of such surveillance mechanism in human cells, we have cloned a full-length cDNA encoding a
T W Seeley et al.
Biochemical and biophysical research communications, 257(2), 589-595 (1999-04-13)
The BUB/MAD signaling pathway monitors attachment of chromosomes to spindle poles in mitotic cells. Mutations of the human BUB1 locus were identified in cancer cells exhibiting an unstable chromosomal complement. We report that the human BUB3 gene maps to a
Min Wu et al.
Nature communications, 10(1), 273-273 (2019-01-19)
Faithful chromosome segregation requires proper chromosome congression at prometaphase and dynamic maintenance of the aligned chromosomes at metaphase. Chromosome missegregation can result in aneuploidy, birth defects and cancer. The kinetochore-bound KMN network and the kinesin motor CENP-E are critical for
Christiane Klebig et al.
The Journal of cell biology, 185(5), 841-858 (2009-06-03)
The kinetochore-bound protein kinase Bub1 performs two crucial functions during mitosis: it is essential for spindle checkpoint signaling and for correct chromosome alignment. Interestingly, Bub1 mutations are found in cancer tissues and cancer cell lines. Using an isogenic RNA interference
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