A1164

Azaserin

Hybri-Max^™, γ-irradiated, 50x, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(e): O-Diazoacetyl-L-serin
• Empirische Formel (Hill-System): C₅H₇N₃O₄
• CAS-Nummer: 115-02-6
• Molekulargewicht: 173.13
• UNSPSC Code: 12352207
• NACRES: NA.75
• PubChem Substance ID: 24890511
• EC Number: 204-061-6
• Beilstein/REAXYS Number: 1726602
• MDL number: MFCD00036802

Eigenschaften

• grade: Hybri-Max^™
• Quality Segment: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• antibiotic activity spectrum: fungi
• mode of action: enzyme | inhibits
• storage temp.: −20°C
• SMILES string: N[C@@H](COC(=O)C=[N+]=[N-])C(O)=O
• InChI: 1S/C5H7N3O4/c6-3(5(10)11)2-12-4(9)1-8-7/h1,3H,2,6H2,(H,10,11)/t3-/m0/s1
• InChI key: MZZGOOYMKKIOOX-VKHMYHEASA-N

Beschreibung

General description

Chemical structure: amino acid derivatives

Application

For use in hybridoma cell culture applications as a myeloma selection agent. Used to study inhibition of formylglycinamide ribonucleotide amidotransferase and PRPP amidotransferase.

Biochem/physiol Actions

Azaserine is an antibiotic, generated by Streptomyces fragilis. It is considered as a potential mutation inducing agent. It is known to promote pancreatic cancer and induces adenoma and carcinoma in vivo and in vitro. Azaserine also stimulates kidney and liver tumors in rats.

Preparation Note

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 5.7 μM azaserine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H301,H351
• pcodes: P201 - P301 + P310 + P330
• Hazard Classifications: Acute Tox. 3 Oral - Carc. 2
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges