This supplement product is tested in growth promotion assays at 10% in medium using fairly robust internals stock cell lines, such as VERO and BHK. The concentration may need to be optimized for other cell lines.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/225/678/p14008.pdf
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14008C
Serum Plus™ Medium Supplement
made with gamma irradiated FBS
Synonym(e):
FBS, FCS, GI FBS, GI FCS, GI Serum supplement, GI sera supplement
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Über diesen Artikel
NACRES:
NA.71
UNSPSC Code:
12352207
Sterility:
γ-irradiated by SER-TAIN™ process (FBS), sterile; sterile-filtered
Form:
solution
Shipped in:
dry ice
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Technischer Dienst
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Unterstützung erhaltenQuality Segment
sterility
γ-irradiated by SER-TAIN™ process (FBS), sterile; sterile-filtered
form
solution
shipped in
dry ice
storage temp.
−20°C
General description
SERUM PLUS™ contains a low level of gamma irradiated Fetal Bovine Serum (FBS) supplemented with proteins that promote nutrient transport, cell attachement, and cell growth.
Application
Serum Plus™ is a medium supplement that can be used as a serum substitute for most cell culture applications. It has been used as a supplement in media for maintenance of bloodstream form Trypanosoma brucei Lister 427.
Features and Benefits
Benefits include less than half of the total protein concentration found in serum, reduced levels of inhibitors such as proteases and viral antibodies, and consistency of lot-to-lot performance due to greater control of the component materials.
Preparation Note
Thawing
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.
Note:
Gentle, periodic swirling of this product is crucial for optimum performance. If a bottle is not periodically swirled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.
Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.
Note:
Gentle, periodic swirling of this product is crucial for optimum performance. If a bottle is not periodically swirled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.
Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.
Legal Information
SER-TAIN is a trademark of Sigma-Aldrich Co. LLC
SERUM PLUS is a trademark of Sigma-Aldrich Co. LLC
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Dieser Artikel | |||
|---|---|---|---|
| description made with gamma irradiated FBS | description Canada origin, sterile-filtered, γ-irradiated, suitable for cell culture | description Supplemented FBS, EU approved | description USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma |
| sterility γ-irradiated by SER-TAIN™ process (FBS), sterile; sterile-filtered | sterility γ-irradiated, sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 500 |
| form solution | form - | form - | form - |
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How much Serum plus medium supplement (14008C) is added to base medium? Is it equivalent to standard FBS (i.e., 10% of volume)?
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