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FCMAB323PE

Sigma-Aldrich

Milli-Mark® Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10®

clone 4G10, Milli-Mark®, from mouse

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Konjugat

PE

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

4G10, monoclonal

Speziesreaktivität

vertebrates, human

Hersteller/Markenname

Milli-Mark®

Methode(n)

flow cytometry: suitable

Isotyp

IgG2bκ

Versandbedingung

wet ice

Posttranslationale Modifikation Target

phosphorylation (pTyr)

Angaben zum Gen

human ... PID1(55022)

Allgemeine Beschreibung

Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Spezifität

Antibody recognizes tyrosine-phosphorylated proteins from all species
Expected to detect tyrosyne phosphorylated proteins in all species.

Anwendung

Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.

Qualität

Evaluated by flow cytometry using EGF treated versus untreated A431 cells

Zielbeschreibung

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Physikalische Form

Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Hinweis zur Analyse

Control
Treated versus untreated A431 cells

Rechtliche Hinweise

4G10 is a registered trademark of Upstate Group, Inc.
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Neel H Shah et al.
eLife, 7 (2018-03-17)
The specificity of tyrosine kinases is attributed predominantly to localization effects dictated by non-catalytic domains. We developed a method to profile the specificities of tyrosine kinases by combining bacterial surface-display of peptide libraries with next-generation sequencing. Using this, we showed
Veronika Horkova et al.
Cell reports, 30(5), 1504-1514 (2020-02-06)
Overtly self-reactive T cells are removed during thymic selection. However, it has been recently established that T cell self-reactivity promotes protective immune responses. Apparently, the level of self-reactivity of mature T cells must be tightly balanced. Our mathematical model and experimental data

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