CP06

Anti-α-Tubulin Mouse mAb (DM1A)

liquid, clone DM1A, Calbiochem^®

• Synonym(e): Mouse Anti-α-Tubulin, α-Tubulin Detection Antibody, Anti-Tubulin DM1A
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: DM1A, monoclonal
• Species reactivity: human, mouse, gerbil, chicken, rat
• Application: —
• Citations: 646

Eigenschaften

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: DM1A, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse, gerbil, chicken, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Immunoblotting (20-100 ng/mL, colorimetric or chemiluminescence; ; Immunofluorescence (5 µg/mL))
• isotype: IgG1
• shipped in: wet ice
• Quality Level: 100
• Gene Information: human ... TUBA1A(7846)
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.

Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Immunogen

Chicken

native chick brain microtubules

Application

Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)

Immunofluorescence (5 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
trp E (Ab-1)

Positive Control
Any cell line

Other Notes

Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable