ABN495

Anti-DARC Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): DARC Antibody, Duffy antigen, Fy glycoprotein, GpFy, Cell adhesion molecule 3, CADM3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse
• Speziesreaktivität (Voraussage durch Homologie): human (immunogen homology)
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt-Hinterlegungsnummer: Q16570
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ACKR1(2532)

Beschreibung

Allgemeine Beschreibung

Duffy antigen/chemokine receptor (DARC), also known as Fy glycoprotein (GpFy), is a multi-pass membrane protein belonging to the G-protein coupled receptor Duffy family. DARC binds, with high affinity, several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. DARC is found in adult kidney, adult spleen, bone marrow, and fetal liver. It is expressed along postcapillary venules throughout the body, except in the adult liver.

Spezifität

This antibody recognizes the C-terminus of DARC.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human DARC.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsneurowissenschaft

Detect Duffy antigen/chemokine receptor using this rabbit polyclonal antibody, Anti-DARC Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected DARC in mouse brain tissue.

Qualität

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DARC in 15 µg of mouse brain tissue lysate.

Zielbeschreibung

~23 and 35 kDa observed. Uniprot describes two isoforms at ~35 and ~36 kDa

Physikalische Form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Mouse brain tissue lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable