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Key Documents

AB5491

Sigma-Aldrich

Anti-Calcium Channel Cav3.1 Antibody

Chemicon®, from rabbit

Synonym(e):

T-type Alpha 1G

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

rat

Hersteller/Markenname

Chemicon®

Methode(n)

western blot: suitable

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Spezifität

Recognizes Cav3.1. The epitope specific for Cav3.1 is not present in any other known proteins.

SPECIES REACTIVITIES:

The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.

Immunogen

Highly purified peptide corresponding to residues 1-22 of rat Cav3.1 (Accession O54898).

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Ionenkanäle & -transporter

Neuroinflammation & Schmerz
This Anti-Calcium Channel Cav3.1 Antibody is validated for use in WB for the detection of Calcium Channel Cav3.1.
Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Physikalische Form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Lagerung und Haltbarkeit

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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H-Sätze

P-Sätze

Gefahreneinstufungen

Aquatic Chronic 3

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Bohumila Jurkovicova-Tarabova et al.
Channels (Austin, Tex.), 12(1), 378-387 (2018-11-08)
Contributions of voltage sensing S4 segments in domains I - IV of CaV3.1 channel to channel activation were analyzed. Neutralization of the uppermost charge in individual S4 segments by exchange of arginine for cysteine was employed. Mutant channels with single

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