09-872

Anti-Methylcytosine dioxygenase TET1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): tet oncogene 1, Ten-eleven translocation 1 gene protein, CXXC-type zinc finger protein 6, methylcytosine dioxygenase TET1, CXXC finger 6, ten-eleven translocation-1, Leukemia-associated protein with a CXXC domain
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse
• Methode(n): ChIP: suitable (ChIP-seq); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_081660
• UniProt-Hinterlegungsnummer: Q3URK3
• Versandbedingung: wet ice
• Angaben zum Gen: human ... TET1(80312)

Beschreibung

Allgemeine Beschreibung

Methylcytosine dioxygenase TET1 (mTET1) is a member of the TET family and contains a characteristic CXXC-type zinc finger domain. mTET1 binds methylcytosine (5mC), a minor, mammalian DNA base necessary to development and retrotransposon silencing, and acts as a catalyst to induce the conversion of 5mC to 5-hydromethylcytosine (hmC or 5hmC), a mammalian DNA base thought to be involved in chromatin structure and in the development of fetal brain, heart and lung tissues. Recent studies indicate that mTET1 regulates the levels of 5hmC, thereby influencing patterns in DNA methylation.

Spezifität

Other homologies: Rat (66% sequence homology), Human (61% sequence homology).

This antibody recognizes methylcytosine dioxygenase TET1 near the C-terminus.

Immunogen

Epitope: Near the C-terminus

KLH-conjugated linear peptide corresponding to mouse Methylcytosine dioxygenase TET1 near the C-terminus.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histon-modifizierende Proteine

Anti-Methylcytosine dioxygenase TET1 Antibody is a Rabbit Polyclonal Antibody for detection of Methylcytosine dioxygenase TET1 also known as tet oncogene 1 & has been validated in WB, ChIP-seq.

Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.

Qualität

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Methylcytosine dioxygenase TET1 in 10 µg of mouse embryonic stem cell lysate.

Zielbeschreibung

~220 kDa observed.
Uncharacterized bands may be seen at ~32, 68, and 70 kDa

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Mouse embryonic stem cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable