Yes. The chaotropic agents and the detergent in the kit will denature the proteins at least partially.
PE0230
Plant Total Protein Extraction Kit
Suitable for any plant species or tissue
Sinónimos:
Total Protein Extraction Kit
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1 KIT
US$ 417,00
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1 KIT
US$ 417,00
About This Item
Código UNSPSC:
41105500
NACRES:
NA.56
Productos recomendados
técnicas
protein extraction: suitable
temp. de almacenamiento
−20°C
Descripción general
The Plant Total Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes two reagents; a plant specific protease inhibitor cocktail and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). Routine use of the reagent in sequential protein extraction of a plant tissue sample with fresh reagent removes nearly all protein from the tissue. The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, metalloproteases, aspartic, and aminopeptidases. It contains pepstatin A, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, leupeptin, 1,10-phenanthroline, and bestatin. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.
Aplicación
Cantidad
Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
Código de clase de almacenamiento
11 - Combustible Solids
Punto de inflamabilidad (°F)
185.0 °F - closed cup
Punto de inflamabilidad (°C)
85 °C - closed cup
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Will my protein samples be denatured when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the expected protein yield when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
Depending on the tissue used (age, type (leaf, stem, root) health, etc.) one can expect 1-4 mg/mL at about 75-80% of the total protein.
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How can I improve my yield when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
A second extraction can be performed and combined with the first to increase protein extracted to about 95% of the total.
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