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M5519

Sigma-Aldrich

Medio basal Murashige y Skoog

powder, suitable for plant cell culture

Sinónimos:

Medio basal MS

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1 L
US$ 37,90
10 L
US$ 124,00
50 L
US$ 186,00

US$ 37,90

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1 L
US$ 37,90
10 L
US$ 124,00
50 L
US$ 186,00

About This Item

Número MDL:
MFCD00240976
Código UNSPSC:
12352207
NACRES:
NA.72

US$ 37,90

PRECIOS SIN IMPUESTOS NACIONALES
Check Cart for Availability
Solicitar un pedido a granel

Formulario

powder

técnicas

cell culture | plant: suitable

aplicaciones

agriculture

temp. de almacenamiento

2-8°C

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Descripción general

El medio Murashige y Skoog se utiliza mucho como medio de cultivo de tejidos vegetales. El medio M&S basal contiene macronutrientes, entre ellos elevadas concentraciones de nitratos y aditivos orgánicos, como agar, azúcares, vitaminas y reguladores del crecimiento. Importantes reguladores del crecimiento agregados con frecuencia al M&S son el IAA (auxina/morfógeno) y la cinetina (citocinina/promotor de la división celular).

Aplicación

El medio Murashige y Skoog se ha utilizado en medios de cultivo de tejido vegetal para estudios de transformación vegetal.[1][2]

Variante de la fórmula

Con macronutrientes y micronutrientes, y vitaminas como describieron Murashige y Skoog (1962)

Formulación de los medios

Cantidad

Formulado para contener 4,4 gramos de polvo por litro de medio.

Nota de preparación

El medio Murashige y Skoog puede reconstituirse a partir de polvo o combinando los productos que constituyen los principales componentes del medio M&S completo, como mezclas de macronutrientes y mezclas de vitaminas. El medio Murashige y Skoog (M5519) contiene los micronutrientes y las vitaminas de la formulación clásica original. Puede complementarse con sacarosa, agar, auxinas (IAA) y citocinas (cinetina) para dar lugar a un medio completo destinado al cultivo de crecimiento de tejido vegetal.

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Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Pictogramas

Flame over circleExclamation mark
GHS03,GHS07

Palabra de señalización

Warning

Frases de peligro

H272,H319

Clasificaciones de peligro

Eye Irrit. 2 - Ox. Sol. 3

Código de clase de almacenamiento

5.1B - Oxidizing hazardous materials

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000422941
0000422941
0000366116
0000366116
0000388916

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Youjun Zhang et al.
Plant communications, 1(5), 100028-100028 (2020-12-29)
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and
Thierry G A Lonhienne et al.
Scientific reports, 4, 5055-5055 (2014-05-24)
Growth, morphogenesis and function of roots are influenced by the concentration and form of nutrients present in soils, including low molecular mass inorganic N (IN, ammonium, nitrate) and organic N (ON, e.g. amino acids). Proteins, ON of high molecular mass
Liang Zhu Y et al.
Plant physiology, 119(1), 73-80 (1999-01-08)
An important pathway by which plants detoxify heavy metals is through sequestration with heavy-metal-binding peptides called phytochelatins or their precursor, glutathione. To identify limiting factors for heavy-metal accumulation and tolerance, and to develop transgenic plants with an increased capacity to
Sina Fischer et al.
Journal of experimental botany, 72(2), 415-425 (2020-10-11)
High Arsenic Concentration 1 (HAC1), an Arabidopsis thaliana arsenate reductase, plays a key role in arsenate [As(V)] tolerance. Through conversion of As(V) to arsenite [As(III)], HAC1 enables As(III) export from roots, and restricts translocation of As(V) to shoots. To probe
Jingyi Wang et al.
Scientific reports, 9(1), 141-141 (2019-01-16)
Abiotic stress significantly impacts growth and yield of crop plants. It is imperative for crop improvement to discover and utilize stress-tolerant functional genes. In this study, genes responding to abiotic stresses, such as freezing, salt and osmotic stress, were screened
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Questions

1–10 of 11 Questions  

  1. How do I prepare this medium?

    1 answer

    1. The preparation instructions for this product are as follows:
      1. Using a container twice the size of the desired final volume, measure out ~90% of the required final volume of tissue culture grade water (e.g., Catalog Number W3500). Example: 900 ml for a final volume of 1000 ml.
      2. While stirring, add the powdered product.
      3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in step 2.
      4. Add desired supplements (e.g., sucrose, gelling agent, auxins, cytokinins).
      Add heat labile constituents after autoclaving.
      5. While stirring, adjust to the desired pH (e.g., 5.7±0.1) using KOH, NaOH, or HCl.
      6. Add additional tissue culture grade water to bring the medium to the final volume.
      7. If a gelling agent is used, heat the solution to clarity while stirring.
      8. Dispense the medium into culture vessels before or after autoclaving according to the application.
      Add heat labile constituents after autoclaving.
      9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121 °C for at least 15 minutes.

      Please see the link below to review this and additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  2. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    1 answer

    1. Product M5524 is the Murashige and Skoog(M&S) Basal Salt Mixture, only. The M5519 products is M&S Basal Medium which also includes the vitamins myo-Inositol, Nicotinic acid and Pyridoxine HCl. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    1 answer

    1. Please see the link below to review the product datasheet, including the full formulation:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      To review additional Murashige and Skoog formulations, please see the link below:

      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

      Helpful?

  4. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    1 answer

    1. Product M5524 Murashige and Skoog(M&S) Basal Salt Mixture, is an ideal alternative to M5519, M&S Basal Medium. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  5. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. Yes, this medium is autoclavable.

      Helpful?

  6. once the media is prepared, what conditions should it be stored in, and how long can it be stored?

    1 answer

    1. The prepared solution is quite stable but should be stored refrigerated. Monitor the salt solution for particulates or turbidity. After the addition of agar and subsequent sterilization, poured plates or vessels should also be stored aseptically in the refrigerator until use. The shelf life of the stored plates or vessels is most often limited by less stable compounds added to the medium. The end user would have to determine the stability of these components. While there is no firm shelf life on the prepared medium, contamination, and drying are the most common reasons for degradation. Monitor plates/vessels for contaminants or turbidity. An indication of excessive drying will be the gelled medium pulling away from the sides of the plate or vessel. The medium has lost moisture and may not be suitable for use. Moisture loss can be minimized by storing prepared plates/vessels in a sealed container or sealed bags. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  7. What is the percentage of sucrose in M5519?

    1 answer

    1. The M5519 does not contain sucrose. This component would have to be added prior to autoclaving. The typical amount is 30 g/L. See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Should sucrose be desired, see product M9274, which is more of a complete formulation containing M&S salts, vitamins, sucrose, and agar. See the link below to review the formulations of the various Murashige & Skoog media:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog#Basal%20Salts%20Mixtures

      Helpful?

  8. What is the quantity and what is shelf life

    1 answer

    1. The SKU sizes indicated refer to the final volume of media that can be made upon reconstitution of the pre-weighted powder. This powdered media is formulated to contain 4.4 grams of powder per liter of medium. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. Product M5519, Murashige and Skoog Basal Medium, has precipitates; will that affect the growth?

    1 answer

    1. Precipitates are known to occur, with time, in plant tissue culture media.  The precipitates have been analyzed.  They are composed of small pale yellow-white particles.  Analysis of precipitates indicated a predominance of iron, phosphate, and zinc.  The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions.  When the solubility of ferric phosphate is exceeded precipitation occurs.  There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.

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1–10 of 11 Questions  

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