This product may be prepared in 10 mM Tris-HCl, pH 8.0 at 10 mg/mL. The material is also soluble in water at 10 mg/mL. Aqueous solutions are stable for at least one month when stored at 2-8 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf
L6876
Lisozima from chicken egg white
powder or granules, ≥39,000 units/mg protein
Sinónimos:
Mucopéptido N-acetilmuramoilhidrolasa, Muramidasa
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1 G
US$ 100,00
5 G
US$ 318,00
10 G
US$ 423,00
25 G
US$ 949,00
100 G
US$ 3.400,00
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1 G
US$ 100,00
5 G
US$ 318,00
10 G
US$ 423,00
25 G
US$ 949,00
100 G
US$ 3.400,00
About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
biological source
chicken egg white
Quality Level
200
300
form
powder or granules
specific activity
≥39,000 units/mg protein
mol wt
single-chain 14.3 kDa
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
storage temp.
room temp
Gene Information
chicken ... LYZ(396218)
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Application
La enzima rompe las paredes celulares de las bacterias; se utiliza para preparar esferoplastos.
Biochem/physiol Actions
La lisozima hidroliza los enlaces β(1→4) entre los restos del ácido N-acetilmurámico y los restos de N-acetil-D-glucosamina en el peptidoglucano y entre los residuos de N-acetil-D-glucosamina en la quitodextrina. Las células grampositivas son bastante sensibles a esta hidrólisis, ya que sus paredes celulares tienen una elevada proporción de peptidoglucanos. Las bacterias gramnegativas son menos sensibles debido a la presencia de una membrana externa y a una menor proporción de peptidoglucanos. Sin embargo, estas células pueden ser hidrolizadas en presencia de EDTA que forma complejos con los iones metálicos en la membrana bacteriana externa.
La enzima es activa en un amplio intervalo de pH (de 6,0 a 9,0). A pH 6,2, se observa actividad máxima en un intervalo de fuerzas iónicas más amplio (de 0,02 a 0,100 M) que a pH 9,2 (de 0,01 a 0,06 M).
La enzima es activa en un amplio intervalo de pH (de 6,0 a 9,0). A pH 6,2, se observa actividad máxima en un intervalo de fuerzas iónicas más amplio (de 0,02 a 0,100 M) que a pH 9,2 (de 0,01 a 0,06 M).
La lisozima hidroliza los enlaces β(1→4) entre los restos del ácido N-acetilmurámico y los restos de N-acetil-D-glucosamina en el peptidoglucano y entre los residuos de N-acetil-D-glucosamina en la quitodextrina. Las células grampositivas son bastante sensibles a esta hidrólisis, ya que sus paredes celulares tienen una elevada proporción de peptidoglucanos.
Features and Benefits
La lisozima de la clara de huevo de pollo es una enzima versátil con propiedades únicas que la hacen eficaz para diversas aplicaciones, entre ellas la degradación de la pared celular bacteriana, la extracción del ADN genómico y como patrón externo para análisis de masa. Es fiable, activa en un amplio intervalo de pH y está disponible en varios tamaños de envase.
Unit Definition
Una unidad producirá un ΔA450 de 0,001 por minuto a pH 6,24 y 25 °C, utilizando una suspensión de Micrococcus lisodeikticus como sustrato, en una mezcla de reacción de 2,6 ml (trayectoria de la luz de 1 cm).
Other Notes
Véase más información sobre enzimas para análisis de carbohidratos complejos en www.sigma-aldrich.com/enzymeexplorer
Application
Referencia del producto
Descripción
Precios
also commonly purchased with this product
Referencia del producto
Descripción
Precios
substrate
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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Artículos
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Protocolos
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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Chromatograms
application for HPLC-
I have ordered this product and stored in -70 degree C. But there is no dilution preparation method available here. Can you please tell me hot to prepare that.
1 answer-
Helpful?
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What's the best buffer and pH for the preparation of lysozyme stock solution, and can I incubate it at room temperature for 1 day?
1 answer-
This product can be resuspended in 350 µL of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl,
1 mM EDTA, and 5% [w/v] TRITON® X-100). The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).During this assay, the end-user should Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Cat. No. T0918) with 25 µg/mL tetracycline (Cat. No. T3383) and 25 µg/mL ampicillin (Cat. No. A9518). The
optimal pH for E. coli cell lysis is 8.0 ± 0.1.16For more information please read through the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Under what conditions can Product L6876, Lysozyme from chicken egg white, be used to lyse E. coli?
1 answer-
The following procedure is for the lysis of E. coli. It may be used as a guideline for other species.1. Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Product No. T 0918) with 25 μg/ml tetracycline (Product No T 3383) and 25 μg/ml ampicillin (Product No. A 9518).2. Centrifuge 1-2 ml samples of the overnight culture.3. Resuspend the pellets in 350 μl of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1 mM EDTA, and 5% [w/v] TRITON® X-100).4. Add 25 μl of a freshly prepared lysozyme solution (500,000 units/ml in 10 mM Tris-HCl, pH 8.0).5. Mix by vortexing for 3 seconds.6. Incubate the lysis mixture for 30 minutes at 37 °C7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds.8. Centrifuge the lysis mixture at 14,000 x g.9. Remove the pellet (cell debris) from the tube using a sterile toothpick.10. Plasmid DNA from the supernatant may then be purified and analyzed.
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What is the amino acid sequence of Product L6876, Lysozyme from chicken egg white?
1 answer-
Lysozyme is a single chain polypeptide of 129 amino acids. The amino acid sequence of chicken egg white Lysozyme is described in the Journal of Biological Chemistry, 238, 2698-2707 (1963).
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How can Product L6876, Lysozyme from chicken egg white, be reconstituted and can stock solutions be prepared?
1 answer-
For the lysis procedure, use a freshly prepared solution of 500,000 units/ml in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should be stable for at least one month when stored between 2-8 °C.
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Can Product L6876, Lysozyme from chicken egg white, be inactivated by heat?
1 answer-
Solutions of Lysozyme may be inactivated by boiling for 40 seconds at 100°C at an alkaline pH.
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