11814320001

Formamida

=99%, liquid, pH 4-10 (20 °C, 200 g/L), suitable for hybridization

• Sinónimos: Amida fórmica, Amidas C₁
• Fórmula lineal: HCONH₂
• Número de CAS: 75-12-7
• Peso molecular: 45.04
• Beilstein/REAXYS Number: 505995
• MDL number: MFCD00007941
• UNSPSC Code: 41116100
• PubChem Substance ID: 329749563

Propiedades

• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• assay: 99%
• form: liquid
• autoignition temp.: 932 °F
• mol wt: 45.04
• packaging: pkg of 500 mL
• manufacturer/tradename: Roche
• technique(s): hybridization: suitable
• color: colorless
• refractive index: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• density: 1.134 g/mL at 25 °C (lit.)
• absorption: ≤0.08 at 290 nm
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Descripción

General description

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.

Application

Formamide has been used in:
in situ hybridization

• as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
• for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
• as a component of stop buffer used in cleavage and polyadenylation activity assays

La formamida desestabiliza los dúplex de ácidos nucleicos y puede utilizarse, normalmente, a una concentración del 50 % en protocolos de hibridación que requieran temperaturas de hibridación mas bajas.

Quality

Contaminants: ≤5 ppm heavy metals (as Pb), ≤5 ppm Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H351,H360D,H373
• pcodes: P201 - P202 - P260 - P280 - P308 + P313 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 1
• flash_point_f: 305.6 °F
• flash_point_c: 152 °C