This product may be stored at room temperature.
47671
Formamida
BioUltra, for molecular biology, ≥99.5% (T)
Sinónimos:
Amida fórmica, Amidas C1
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
250 ML
US$ 214,00
1 L
US$ 669,00
2.5 L
US$ 1.230,00
Seleccione un Tamaño
Cambiar Vistas
250 ML
US$ 214,00
1 L
US$ 669,00
2.5 L
US$ 1.230,00
About This Item
Fórmula lineal:
HCONH2
Número de CAS:
Peso molecular:
45.04
Beilstein/REAXYS Number:
505995
EC Number:
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.25
grade:
for molecular biology
assay:
≥99.5% (T)
technique(s):
FISH: suitable
hybridization: suitable
protein extraction: suitable
hybridization: suitable
protein extraction: suitable
application(s):
genomic analysis
bp:
210 °C (lit.)
vapor pressure:
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
30 mmHg ( 129 °C)
Productos recomendados
grade
for molecular biology
Quality Level
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
product line
BioUltra
assay
≥99.5% (T)
form
liquid
autoignition temp.
932 °F
technique(s)
FISH: suitable
hybridization: suitable
protein extraction: suitable
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
ign. residue
≤0.1%
refractive index
n20/D 1.447 (lit.)
n20/D 1.447
pH
7-9 (25 °C, 1 M in H2O)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
solubility
H2O: 10 M at 20 °C, clear, colorless
density
1.134 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg
cation traces
Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg
SMILES string
NC=O
λ
10 M in H2O
UV absorption
λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1
application(s)
genomic analysis
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
Inchi Key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
¿Está buscando productos similares? Visita Guía de comparación de productos
General description
Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.
Application
Formamide has been used:
- as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
- in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
- in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
- to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
- as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent
La formamida desestabiliza los dúplex de ácidos nucleicos y puede utilizarse, normalmente, a una concentración del 50 % en protocolos de hibridación que requieran temperaturas de hibridación mas bajas.
Biochem/physiol Actions
Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.
Features and Benefits
- Versatile and adaptable for a wide variety of laboratory and research applications
- BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations
Other Notes
For additional information on our range of Biochemicals, please complete this form.
signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
-
What temperature should this product be stored at?
1 answer-
Helpful?
-
-
What temperature should this product be stored at?
1 answer-
This product may be stored at room temperature.
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
-
-
What expiry does Formamide get?
1 answer-
The product specification sheet for this product contains a recommended retest date period of roughly 11 months after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
Helpful?
-
-
What is the best way to deionize Formamide, Product 47671?
1 answer-
Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.
Helpful?
-
-
Are aqueous solutions of Formamide, Product 47671, stable?
1 answer-
In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).
Helpful?
-
-
What is the molarity of the neat liquid Formamide, Product 47671?
1 answer-
Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
