Skip to Content
Merck
All Photos(1)

Documents

34877

Sigma-Aldrich

Water

HPLC Plus, suitable for HPLC, suitable for SM 4500 - NH3

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
UNSPSC Code:
41116105
PubChem Substance ID:
329755083
NACRES:
NA.21

product name

Water, HPLC Plus

grade

HPLC Plus

Quality Level

100

Agency

suitable for SM 4500 - NH3

vapor density

<1 (vs air)

vapor pressure

3 mmHg

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable

impurities

≤0.0003% non-volatile matter
≤1 ppb fluorescence (quinine) at 254 nm
≤1 ppb fluorescence (quinine) at 365 nm

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

anion traces

chloride (Cl-): ≤0.1 mg/kg
fluoride (F-): ≤0.1 mg/kg
nitrate (NO3-): ≤0.1 mg/kg
sulfate (SO42-): ≤0.1 mg/kg

HPLC-gradient

≤1 mAU at 254 nm
≤5 mAU at 210 nm

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

HPLC Plus grade water is useful for the UHPLC (Ultra-High Pressure Liquid Chromatography) gradient elution and HPLC studies.

Application

Water has been used in the following studies:
  • To prepare the diluted hydrochloric acid solutions required for the preparation of standard solutions of amino acid.
  • In the sample extraction step during the gas chromatography mass spectrometric (GC-MS) analysis of free (FAA) and combined amino acids (CAA) in aerosol samples.
  • Preparation of tryptic digests from the standard protein samples and milk digests from milk samples.
  • Preparation of C60-aminopropylsilica.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
  • The article number 34877-4X2.5L will be discontinued. Please order the single bottle 34877-2.5L which is physically identical with the same exact specifications.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Manolis Mandalakis et al.
Journal of chromatography. A, 1217(1), 143-150 (2009-12-08)
The analysis of amino acids by gas chromatography mass spectrometry (GC-MS) after their derivatization with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide was investigated as an alternative approach for the determination of free (FAA) and combined amino acids (CAA) in aerosols. This technique showed excellent linearity
Martin Fischnaller et al.
Analytica chimica acta, 761, 92-101 (2013-01-15)
Phosphorylation of proteins is an important cellular regulatory process. The analysis of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of phosphorylated species. Mass spectrometry (MS) of phosphopeptides obtained from tryptic protein digests is
Susu Duan et al.
Nature communications, 5, 5029-5029 (2014-10-10)
Oseltamivir-resistant H1N1 influenza viruses carrying the H275Y neuraminidase mutation predominated worldwide during the 2007-2009 seasons. Although several neuraminidase substitutions were found to be necessary to counteract the adverse effects of H275Y, the order and impact of evolutionary events involved remain
Mary A Rodgers et al.
The Journal of experimental medicine, 211(7), 1333-1347 (2014-06-25)
Linear ubiquitination is a newly discovered posttranslational modification that is currently restricted to a small number of known protein substrates. The linear ubiquitination assembly complex (LUBAC), consisting of HOIL-1L, HOIP, and Sharpin, has been reported to activate NF-κB-mediated transcription in
Yasushi Itoh et al.
PLoS pathogens, 10(6), e1004192-e1004192 (2014-06-20)
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype often cause severe pneumonia and multiple organ failure in humans, with reported case fatality rates of more than 60%. To develop a clinical antibody therapy, we generated a human-mouse chimeric
View All Related Papers

Articles

UHPLC Analysis of Structurally Similar Compounds: A Case Study of Ephedrine and Related Analogs on a Carbon Column

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

UHPLC Analysis of Structurally Similar Compounds: A Case Study of Ephedrine and Related Analogs on a Carbon Column

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

UHPLC Analysis of Structurally Similar Compounds: A Case Study of Ephedrine and Related Analogs on a Carbon Column

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

UHPLC Analysis of Structurally Similar Compounds: A Case Study of Ephedrine and Related Analogs on a Carbon Column

Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.

Protocols

HPLC Analysis of Purified Adeno-Associated Virus (AAV) on TSKgel® SP-NPR

HPLC Analysis of Purified Adeno-associated Virus (AAV) on TSKgel® SP-NPR

HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

Cationic Polymers HPLC on TSKgel® PWXL-CP Columns

Separation of various acids and compounds by molecular weight: PAA, PEI, PIDADMACl, PAS, cationic dextran, chitosan

Cationic Polymers HPLC on TSKgel® PWXL-CP Columns

Separation of various acids and compounds by molecular weight: PAA, PEI, PIDADMACl, PAS, cationic dextran, chitosan

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service