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E7510

Sigma-Aldrich

Earle′s Balanced Salt Solution 10x

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Earle’s Balanced Salts, EBSS

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L

shipped in

ambient

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General description

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Application

Earle′s Balanced Salt Solution 10x has been used:
  • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
  • in the swim-up method to prepare human sperm sample
  • in the steady-state medium to culture bovine primary mammary epithelial cells

Features and Benefits

  • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
  • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
  • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yanli Ren et al.
Cancer letters, 390, 126-136 (2017-01-22)
Dyregulation of autophagy is implicated in human cancers and the mechanism details remains largely unclear. Herein we report the regulatory role of miR-638 in autophagy of esophageal squamous cell carcinoma (ESCC) and breast cancer cells. We found that miR-638 overexpression
Siwen Yang et al.
Food & function, 11(1), 424-434 (2019-12-13)
Insulin resistance is the main cause of type 2 diabetes, fatty liver and obesity. Our previous study found that mushroom polysaccharides improved insulin resistance in vitro, but the underlying mechanisms were still unknown. Thus, we investigate the hypoglycemic effects of
A L Molan et al.
The Veterinary record, 150(3), 65-69 (2002-02-12)
The effects of condensed tannins extracted from seven forages on the viability of the eggs and first stage (L1) larvae of the sheep nematode Trichostrongylus colubriformis were evaluated in in vitro assays. The extracts of condensed tannins were obtained from
Zhenlu Li et al.
Cell death & disease, 9(5), 483-483 (2018-05-01)
Autophagy is an essential cytoprotective response against pathologic stresses that selectively degrades damaged cellular components. Impaired autophagy contributes to organ injury in multiple diseases, including ischemia/reperfusion (I/R), but the exact mechanism by which impaired autophagy is regulated remains unclear. Several
Ming Y Lee et al.
EMBO reports, 18(1), 48-60 (2016-11-10)
PEX13 is an integral membrane protein on the peroxisome that regulates peroxisomal matrix protein import during peroxisome biogenesis. Mutations in PEX13 and other peroxin proteins are associated with Zellweger syndrome spectrum (ZSS) disorders, a subtype of peroxisome biogenesis disorder characterized

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