GE17-5079-02

HiTrap^® rProtein A Fast Flow

Cytiva 17-5079-02, pack of 2 × 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: recombinant protein A (E. coli)
• packaging: pack of 2 × 1 mL
• manufacturer/tradename: Cytiva 17-5079-02
• storage condition: (20% Ehtanol); (20% Ethanol)
• parameter: <4 mL/min flow rate; 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: 4% cross-linked agarose
• particle size: 60-165 μm
• average diameter: 90 μm (d50v)
• cleaning: 2-11
• working range: 3-10
• capacity: ~50 mg binding capacity (human lgG/ml medium)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

Features and Benefits

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established Protein A-based medium used in many approved MAb processes.
• Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA^® design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids