GE17-5080-01
HiTrap® rprotein A Fast Flow
Cytiva 17-5080-01, pack of 1 × 5 mL
Synonym(s):
HiTrap rProtein, Protein A Fast Flow Column, rProtein A Fast Flow
About This Item
Recommended Products
ligand
recombinant protein A (E. coli)
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 17-5080-01
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
4% cross-linked agarose
particle size
60-165 μm
average diameter
90 μm (d50v)
cleaning
2-11
working range
3-10
capacity
~50 mg binding capacity (human lgG/ml medium)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
Features and Benefits
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
Storage and Stability
Analysis Note
Other Notes
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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