CRISPRi & CRISPRa Genetic Screening

Lentiviral Libraries and Pools for CRISPR Activation and Inhibition

Discover our optimized CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Our pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized. Individual guide RNAs for both systems are also easily ordered online.

Sigma-Aldrich^® Advanced Genomics CRISPRi and CRISPRa libraries complement our comprehensive suite of genome engineering technologies to allow you to uncover further insights into complex biological processes. Large scale LOF (loss-of-function) and GOF (gain-of-function) screens can identify unique, yet functionally related, gene pathways and hits which are often missed under otherwise identical KO (knockout) screening parameters. Take your experiments Beyond the Bench with the largest genomics portfolio available and a partner that can ensure your experimental goals are met.

Specifications

CRISPRi	CRISPRa
~10 gRNAs/gene ~258,000 total clones Targets the human genome Lentivirus provided as 200 µL (8 x 25 µuL) aliquots at a minimum of 5x108 TU/mL Off-the-shelf subpools available	~3 gRNAs/gene ~70,000 total clones Targets human and mouse genomes Lentivirus provided as 200 µL (8 x 25 uµLl) aliquots at a minimum of 5x108 VP/mL Custom subpools available

The Sigma-Aldrich^® CRISPR inhibition libraries have superior algorithm placement and improved sgRNA scaffolding for optimal gene knockdown, even in the most difficult targets.

Added Benefits:

• We are a 10x Genomics compatible partner with ready-to-use custom CRISPR lenti pools with Feature Barcode technology included in our vectors to enable discovery at single-cell resolution
• Individual CRISPR inhibition & activation guides can be easily ordered online – predesigned or custom
• In house DNA Prep and Deconvolution Services

Figure 1. CRISPRi and CRISPRa utilize catalytically inactive dCas9 to recruit the transcriptional repressor KRAB domain or the transcriptional activators HSF1, p65 and MS2 to silence and overexpress target gene expression respectively.

Not sure which screening tool is right for your experiment? Pricing questions? Need a custom pool? We can help!

Resources

CRISPRi User Manual
CRISPRi FAQ
CRISPRi Data Sheet
10x Data Sheet
CRISPRa User Manual

Lentivirus Protocols
CRISPRa Data Sheet
10x Genomics Protocol
Screening Overview
CRISPRi/a Product Guide

Vector Maps

SAM CRISPRa

CRISPRi

CRISPRi Available Libraries and Pools

Product Description	Target	Number of Genes	Product Number
Whole-Genome Libraries
Human Whole Genome & Non-Coding CRISPRi Library	Human, Whole genome	18,905	CRISPRILIB
Control & Effector Kit: Positive & Negative controls with dCas9	Coding & non-targeting	Coding gene RAB1A and a non-targeting control	CRISPRICON
CRISPRi Subpools
10x Genomics Feature Barcode Optimization Kit	Human, Coding	Coding gene RAB1A and a non-targeting control	CRISPRI10X
Druggable Genome Pool	Human, Coding	2,318	CRISPRIS01
Cancer & Apoptosis Pool	Human, Coding	2,916	CRISPRIS02
Stress Proteostasis Pool	Human, Coding>	3,093	CRISPRIS03
Mitochondria/Trafficking/Motility Pool	Human, Coding	2,219	CRISPRIS04
Gene Expression Pool (Transcription Factors)	Human, Coding	2,292	CRISPRIS05
Membrane Proteins Pool	Human, Coding	2,418	CRISPRIS06
Unassigned Pool (Uncategorized Gene Function, Novel Targets)	Human, Coding	1,824	CRISPRIS07
Common Cancer Cell Lines Pool & iPSC Pool: Common to 7 cell lines (5 cancer, iPSC, HFF)	Human, Long non-coding	691	CRISPRIS08
iPSC Pool	Human, Long non-coding	3,000	CRISPRIS09
Common Cancer Cell Lines Pool (HeLa, HEK293T, K562, MCF7, U87 cell lines)	Human, Long non-coding	1,329	CRISPRIS10

SAM CRISPRa Libraries and Helper Constructs

Product Description	Target	Number of Genes	Product Number
Whole-Genome SAM Libraries
SAM CRISPRa Library Zeo	Human, coding	23,430	HSAMZEO
SAM CRISPRa Library Puro	Human, coding	23,430	HSAMPURO
SAM CRISPRa Library Zeo	Mouse, coding	23,439	MSAMZEO
SAM CRISPRa Library Puro	Mouse, coding	23,439	MSAMPURO
SAM Helper Constructs: Plasmid DNA
dCas9-VP64-Blasticidin SAM Helper Construct 1	Mammalian		SAMVP64BST
MS2-P65-HSF1-Hygromycin SAM Helper Construct 2	Mammalian		SAMMS2HYG
SAM Helper Construct Kit	Mammalian		SAMHELPERP
SAM Helper Constructs: Lentivirus
dCas9-VP64-Blasticidin SAM Helper Construct 1	Mammalian		SAMVP64BSTV
MS2-P65-HSF1-Hygromycin SAM Helper Construct 2	Mammalian		SAMMS2HYGV
SAM Helper Construct Kit	Mammalian		SAMHELPERV

 

CRISPR Screening Tips

A simple modification to improve CRISPR-pooled screenin result. Reduce NGS noise by eliminating artifact DNA.