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910147

Fluorescein Azide

≥95%

Synonym(s):

4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoesäure, 6-Carboxyfluorescein Azide, 6-FAM azide, N-(3-azidopropyl)-3′,6′-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamide

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About This Item

Empirical Formula (Hill Notation):
C24H18N4O6
CAS Number:
1386385-76-7
Molecular Weight:
458.42
UNSPSC Code:
12352125
NACRES:
NA.22
MDL number:
MFCD18426596
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assay

≥95%

form

powder

reaction suitability

reaction type: click chemistry

manufacturer/tradename

(BCFA-001)

storage temp.

−20°C

SMILES string

[N+](=[N-])=NCCCNC(=O)c1cc(c(cc1)C(=O)O)C2=C3C(=CC(=O)C=C3)Oc4c2ccc(c4)O

InChI

1S/C24H18N4O6/c25-28-27-9-1-8-26-23(31)13-2-5-16(24(32)33)19(10-13)22-17-6-3-14(29)11-20(17)34-21-12-15(30)4-7-18(21)22/h2-7,10-12,29H,1,8-9H2,(H,26,31)(H,32,33)

InChI key

SJAGNCADRVABJA-UHFFFAOYSA-N

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This Item
B8806A4108D1946
Fluorescein Azide ≥95%

Sigma-Aldrich

910147

Fluorescein Azide

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 1 mg/mL in DMSO

Sigma-Aldrich

B8806

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

Sigma-Aldrich

Sigma-Aldrich

A4108

Aminophenyl fluorescein solution

DAF-FM DA ≥98% (HPLC)

Sigma-Aldrich

D1946

DAF-FM DA

form

powder

form

liquid

form

liquid

form

powder

assay

≥95%

assay

-

assay

-

assay

≥98% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

reaction suitability

reaction type: click chemistry

reaction suitability

-

reaction suitability

-

reaction suitability

-

manufacturer/tradename

(BCFA-001)

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

Application

This 488nm absorbing dye is probably the most popular dye in cell biology and represents an alternative to Alexa Fluor 488 and the DyLight 488. Fluorescent dye azides can be used for the fluorescent labeling of terminal alkyne-modified molecules by the Cu(I)-catalyzed azide-alkyne (CuAAC) reaction (click reaction).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKDB0021
MKCN9304
MKCM7954
MKCM3649

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Click-click-click: single to triple modification of DNA.
Philipp M E Gramlich et al.
Angewandte Chemie (International ed. in English), 47(18), 3442-3444 (2008-04-03)
Philipp M E Gramlich et al.
Angewandte Chemie (International ed. in English), 47(44), 8350-8358 (2008-09-25)
The attachment of labels onto DNA is of utmost importance in many areas of biomedical research and is valuable in the construction of DNA-based functional nanomaterials. The copper(I)-catalyzed Huisgen cycloaddition of azides and alkynes (CuAAC) has recently been added to

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. What can I dissolve fluorescein azide in?

    1 answer

    1. This product has not been tested for solubility. However, external sources indicate that this compound is soluble in DMSO, DMF, and MeOH at 10 mM. This information has not been validated.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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