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M1778

Mucin from porcine stomach

Type III, bound sialic acid 0.5-1.5 %, partially purified powder

Synonym(s):

MUC

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10 g

Available to ship TODAYfromMILWAUKEE

$109.00
100 g

Available to ship TODAYfromMILWAUKEE

$776.00

About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
282-010-7
NACRES:
NA.61
MDL number:
Form:
partially purified powder
Biological source:
Porcine stomach

$109.00


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biological source

Porcine stomach

Quality Segment

type

Type III

form

partially purified powder

composition

bound sialic acid, 0.5-1.5%

technique(s)

microbiological culture: suitable

solubility

NaOH: soluble 20 mg/mL

storage temp.

2-8°C

General description

Mucins or mucus glycoproteins are the main macromolecular components of mammalian mucus.[1] It was also used in a study to evaluate the sputum smears concentrated by cyto-centrifugation for detection of acid-fast bacilli.[2]

Application

Mucin from porcine stomach was used in studies on the binding site of the galactose-specific agglutinin PA-IL from Pseudomonas aeruginosa.[3]

Biochem/physiol Actions

Mucus forms the protective cover for all epithelial surfaces. The major structural component of mucus is gel-forming mucins. The mucus layer covering the intestinal epithelium has the MUC2 mucin as its central molecule. MUC2 is produced by goblet cells.[4]

Preparation Note

Prepared according to the method of Glenister, et al., for use in complex growth media for dental plaque bacteria.

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This Item
M3895G2625A1830
biological source

Porcine stomach

biological source

bovine submaxillary glands

biological source

Porcine skin

biological source

Porcine

technique(s)

microbiological culture: suitable

technique(s)

electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

partially purified powder

form

powder

form

powder

form

lyophilized powder

solubility

NaOH: soluble 20 mg/mL

solubility

-

solubility

H2O: soluble 50 mg/mL

solubility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Articles

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Global Trade Item Number

SKUGTIN
M1778-100G04061834041108
M1778-10G04061835557660

Questions

1–7 of 7 Questions  
  1. Can I reconstitute the mucin so that it forms a saliva/snot-like substance? I can't seem to find any papers that have done this...

    1 answer
    1. Mucins are soluble in water, forming hazy viscous solutions.

      Helpful?

  2. What is the molecular weight of Product M1778, Mucin from porcine stomach?

    1 answer
    1. This product is partially purified from crude mucin, M2378.  Sigma has not determined the molecular weight of this product. Neither has the sugar and amino acid composition been determined.

      Helpful?

  3. Is Product M1778, Mucin from porcine stomach, a purified preparation of mucin?

    1 answer
    1. This product is a partially purified preparation of mucin based on the method of Glenister cited on the product's webpage.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. How can I solubilize Product M1778, Mucin from porcine stomach?

    1 answer
    1. Mucin is not readily soluble in aqueous solutions. Turbid "solutions" will form at about 5 mg/mL in 0.1 M acetate buffer, pH 5.0. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.

      Helpful?

  6. What does the "Type" designation in Mucin from porcine stomach mean?

    1 answer
    1. The type designation does not have any particular biological significance.  It is used to emphasize differences in the listings of this product.

      Helpful?

  7. What are the differences between Mucin from porcine stomach Products M2378 and M1778?

    1 answer
    1. Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website.

      Helpful?

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