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B3679

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonym(s):

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

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100 ML
$236.54

$236.54

List Price$256.00Save 8%
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100 ML
$236.54

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

$236.54

List Price$256.00Save 8%
Web-Only Promotion

Available to ship onMay 01, 2025Details

Product Name

BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate

Quality Level

100

form

liquid

storage temp.

2-8°C

Related Categories

Application

BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
  • for the detection of secondary antibodies by immunoblot[1]
  • in ELISPOT assay[2]
  • in dot blot analysis[3]
  • in western blot to visualize protein bands on nitrocellulose membrane[4]

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.[4]

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCH2415
MKCH2414
MKCH2413
MKCF9800
MKCF9801

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T W J M Van Herpen et al.
Annals of botany, 102(3), 331-342 (2008-07-16)
Alpha-gliadin proteins are important for the industrial quality of bread wheat flour, but they also contain many epitopes that can trigger celiac (coeliac) disease (CD). The B-genome-encoded alpha-gliadin genes, however, contain very few epitopes. Controlling alpha-gliadin gene expression in wheat
Membrane bioreactor separator system for integrated IgG fragmentation and Fab purification
Yu D and Ghosh R
Journal of Immunological Methods, 359(1-2), 37-41 (2010)
Characterization of the 41 kDa allergen Asp v 13, a subtilisin-like serine protease from Aspergillus versicolor
Shi C and Miller JD
Molecular Immunology, 48(15-16), 1827-1834 (2011)
Targeting influenza virus hemagglutinin to Xcr1+ dendritic cells in the absence of receptor-mediated endocytosis enhances protective antibody responses
Gudjonsson A, et al.
Journal of Immunology, 198(7), 2785-2795 (2017)
pSTAT5 and ERK exhibit different expression in myeloproliferative neoplasms
Wisniewska-Chudy E, et al.
Oncology Reports, 37(4), 2295-2307 (2017)
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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