S2890
Streptavidin−Alkaline Phosphatase from Streptomyces avidinii
lyophilized powder, ≥700 DEA units/mg protein
Synonym(s):
Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii
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250 μG
$108.75
1 MG
$303.40
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250 μG
$108.75
1 MG
$303.40
About This Item
Recommended Products
conjugate
alkaline phosphatase conjugate
Quality Level
form
lyophilized powder
specific activity
≥700 DEA units/mg protein
composition
Protein, ≥40% biuret
storage temp.
−20°C
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General description
Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin.[1] Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.[2]
Application
Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.[3][4][5]
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.
Unit Definition
One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
Physical form
Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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