Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

B5655

Sigma-Aldrich

BCIP®/NBT

SIGMAFAST™ BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

Synonym(s):

BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate

Sign Into View Organizational & Contract Pricing

Select a Size

5 TABS
$62.50
25 TABS
$219.00

$62.50

In StockDetails

All Photos(1)

Select a Size

Change View
5 TABS
$62.50
25 TABS
$219.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

$62.50

In StockDetails

Product Name

SIGMAFAST™ BCIP®/NBT, tablet

form

tablet

Quality Level

200

solubility

water: 10 mL, clear, yellow

storage temp.

−20°C

Related Categories

Application

SIGMAFAST BCIP®/NBT has been used as a substrate
  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting[1]
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts[2]
  • for alkaline phosphatase staining in bone marrow stromal cells[3]

Reconstitution

Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000377037
0000377040
0000297791
0000293916
0000293917

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
Nicolas Leventoux et al.
Stem cell research, 47, 101896-101896 (2020-07-14)
Amyotrophic Lateral Sclerosis is the most common motor neuron degenerative disease in adults, and TARDBP gene mutations have been reported to be involved in the pathogenesis. We present here how we generated the human induced pluripotent stem cell (hiPSC) line
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Aneela Yasmeen et al.
Molecular biotechnology, 58(12), 807-820 (2016-10-21)
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct
View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Protocols

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

1 Review This action will navigate to reviews.

Questions

  1. Is it ok to store this tablet (sigmafast BCIP/NBT) after being dissolved in water? (in case I couldn't use all 10 mL) If it's ok, which temperature (and other conditions) should I store it?

    1 answer

    1. For best results, it is recommended to use the solution within 1 hour. Long term storage of the solution has not been evaluated. A prepared BCIP/NBT solution is available under product number B6404. This product is prepared in Tris buffer and may be stored at room temperature. See the links below for the product datasheets for each item.

      B5655 Tablets
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/403/473/b5655dat.pdf

      B6404 Premixed Solution
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/719/b6404bul.pdf

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service