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Application
BCIP/NBT solution, premixed has been used:
- as a substrate to develop dot-blots
- as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
- to detect specific immune complexes in western blot analysis
Other Notes
0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2
Substrates
Substrate for visualizing alkaline phosphatase conjugates in Western blotting.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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