NXTRACT
NuCLEAR™ Extraction Kit
For mammalian tissue or cultured cells
Synonyme(s) :
Nuclear Isolation Kit
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About This Item
Code UNSPSC :
41105517
Nomenclature NACRES :
NA.56
Produits recommandés
Niveau de qualité
Utilisation
kit sufficient for 10 extractions (1 ml packed cell volume)
kit sufficient for 100 extractions (100 μl packed cell volume)
Technique(s)
protein extraction: suitable
western blot: suitable
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.
Application
NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.
Autres remarques
Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.
Informations légales
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- 3× Dilution and Equilibration Buffer 90 mL
- P8340Protease Inhibitor Cocktail 1 mLFDS
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Point d'éclair (°F)
188.6 °F - closed cup
Point d'éclair (°C)
87 °C - closed cup
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Les clients ont également consulté
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American journal of physiology. Regulatory, integrative and comparative physiology, 302(9), R1025-R1033 (2012-03-10)
We previously reported that excess of deoxycorticosterone-acetate (DOCA)/salt-induced cardiac hypertrophy in the absence of hypertension in one-renin gene mice. This model allows us to study molecular mechanisms of high-salt intake in the development of cardiovascular remodeling, independently of blood pressure
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