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CELLYTPN1

Sigma-Aldrich

CelLytic™ PN Isolation/Extraction Kit

For plant leaves

Synonyme(s) :

Cell lysis kit

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About This Item

Code UNSPSC :
41116134
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Utilisation

 kit sufficient for 30 extractions (20 g of fresh or frozen leaves)

Technique(s)

ChIP: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

CelLytic™-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.

Application

The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.

Caractéristiques et avantages

Efficient Nuclei Isolation and Protein Extraction: CelLytic™ PN Isolation/Extraction Kit provides a fast and efficient method for isolating nuclei and extracting functional nuclear proteins from plant leaves, saving time and effort for academic researchers.

Versatility: The isolated nuclei can be used as a source for chromatin, genomic DNA, RNA, etc., making CelLytic™ PN Isolation/Extraction Kit a versatile solution for plant molecular biology research.

Suitable for Detection of DNA-Protein Interactions: The nuclear protein extract obtained using this kit is ideal for detecting DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis, western blot assay, and similar techniques, which is crucial for researchers studying transcription factors and chromatin remodeling.

Plant Model Specific Protocol: The CelLytic™ PN Isolation/Extraction Kit offers a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis, making it an easy-to-follow solution for researchers working with these plant models.

Rapid Extraction: The CelLytic™ PN Isolation/Extraction Kit provides academic researchers with a rapid extraction method, allowing them to quickly extract functional nuclear proteins from plant leaves for their research.

Autres remarques

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Informations légales

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Pictogrammes

CorrosionExclamation markEnvironment
GHS05,GHS07,GHS09

Mention d'avertissement

Danger

Mentions de danger

H302,H315,H318,H410

Classification des risques

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Guoyin Liu et al.
Plant physiology and biochemistry : PPB, 156, 155-166 (2020-09-20)
WRKY transcription factors play key roles in plant biotic and abiotic stress responses, but the function of some MaWRKYs remains elusive. Here, we characterized the positive role of MaWRKY80 in drought stress resistance and the underlying mechanism. MaWRKY80 was significantly
Xiaosa Xu et al.
Developmental cell, 56(4), 557-568 (2021-01-06)
Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear. A comprehensive understanding of development requires insight into the full diversity of cell types and developmental domains and the gene networks required
Lei Wang et al.
The EMBO journal, 29(11), 1903-1915 (2010-04-22)
Many core oscillator components of the circadian clock are nuclear localized but how the phase and rate of their entry contribute to clock function is unknown. TOC1/PRR1, a pseudoresponse regulator (PRR) protein, is a central element in one of the
Andrew Farmer et al.
Molecular plant, 14(3), 372-383 (2021-01-11)
Similar to other complex organisms, plants consist of diverse and specialized cell types. The gain of unique biological functions of these different cell types is the consequence of the establishment of cell-type-specific transcriptional programs. As a necessary step in gaining
Rwitie Mallik et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1863(12), 194644-194644 (2020-10-18)
AtHMGB15 belongs to a group of ARID-HMG proteins which are plant specific. The presence of two known DNA binding domains: AT rich interacting domain (ARID) and High Mobility Group (HMG)-box, in one polypeptide, makes this protein intriguing. Although proteins containing
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