NUC101
Nuclei Isolation Kit: Nuclei EZ Prep
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Synonyme(s) :
Rapid mammalian nuclei isolation kit
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32
Produits recommandés
Utilisation
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Durée de conservation
1 yr at 2‑8 °C
Conditionnement
pkg of 1 kit
Application(s)
cell analysis
Activité étrangère
nuclease and protease, free
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.
Application
Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
Actions biochimiques/physiologiques
The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
Autres remarques
All procedures should be carried out on ice or 2-8°C.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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