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B4500

Sigma-Aldrich

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Sinónimos:

BAEE

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10 G
$185.00
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$401.00

$185.00


Fecha estimada de envío17 de abril de 2025



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10 G
$185.00
25 G
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About This Item

Fórmula empírica (notación de Hill):
C15H22N4O3 · HCl
Número de CAS:
Peso molecular:
342.82
Beilstein/REAXYS Number:
3781694
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

$185.00


Fecha estimada de envío17 de abril de 2025


Nombre del producto

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

Quality Level

assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

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General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin.[1] It is a nonprotein substrate used in spectroscopic assays.[2]

Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles[3]
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts[4]
  • in trypsin activity assay of the tail tendon fascicles from rat[5]

Substrates

The prototype substrate for trypsin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation
Bright R, et al.
Clinical Oral Investigations, 22(1), 487-493 (2018)
Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products
Thomas GE, et al.
Process Biochemistry (Oxford, United Kingdom), 44(1), 77-82 (2009)
Yuchen Li et al.
Nutrients, 12(3) (2020-03-22)
Arthrospira platensis (spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic
Paul Kelly et al.
Veterinary research communications, 44(1), 29-39 (2020-02-23)
Isolation and culture of distinct primary endometrial cells are key to reliable in-vitro models to investigate the uterine immune response and optimse new disease interventions. Details on the isolation method and purity of distinct cell populations is lacking in currently
Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)

Artículos

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Protocolos

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

Questions

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