This product is soluble in 1mM HCL.
T6567
Tripsina from porcine pancreas
Proteomics Grade, BioReagent, Dimethylated
Sinónimos:
Porcine Trypsin, Trypsin for Mass Spectropetry
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
20 μG
$65.20
5 X 20 μG
$92.90
1 MG
$859.00
$65.20
Envío en 2 semanas. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)
Solicitar un pedido a granel
Seleccione un Tamaño
Cambiar Vistas
20 μG
$65.20
5 X 20 μG
$92.90
1 MG
$859.00
About This Item
$65.20
Envío en 2 semanas. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)
Solicitar un pedido a granel
Productos recomendados
biological source
Porcine pancreas
Quality Level
product line
BioReagent
solubility
1 mM HCl: soluble 1 mg/mL, clear, colorless
shipped in
wet ice
storage temp.
2-8°C
General description
La tripsina es una proteasa de serina que se utiliza generalmente en bioquímica y biología como un reactivo enzimático importante.[1] Este método produce un producto de tripsina muy purificado adecuado para la investigación en proteómica. Grado proteómica ideal para su uso tanto en soluciones como en hidrolizados de tripsina en gel. La tripsina, una proteasa de serina, está presente en el sistema digestivo de varios vertebrados.[2]
Application
La tripsina del páncreas porcino se utiliza para las siguientes aplicaciones:
- Digestión proteica en gel y análisis mediante espectrometría de masas con MALDI-TOF[3]
- Análisis mediante espectrometría de masas de ionización por electrospray (ESI-MS)[4]
- Perfilado del proteoma de superficie de L. plantarum[5]
- Filtración en gel, ultracentrifugación y microscopia electrónica de sombras rotatorias[6]
- Espectrometría de masas[7][8]
Biochem/physiol Actions
La tripsina se utiliza habitualmente en la investigación proteómica para la cartografía de péptidos y el trabajo de secuenciación de proteínas, debido a su escisión muy específica que produce un número limitado de péptidos tripsínicos. Hidroliza los enlaces peptídicos específicamente en el lado carboxilo de los restos de arginina y lisina.[2] La enzima exhibe también actividades esterasa y amidasa.[9] La tripsina actúa como una herramienta de cultivo celular. Se utiliza para hidrolizar proteínas alergénicas y producir leche hipoalergénica en la industria.[1]
related product
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Shaoming Mao et al.
Molecular bioSystems, 7(5), 1660-1677 (2011-03-09)
The solventogenic bacterium Clostridium acetobutylicum is the most important species of Clostridium used in the fermentation industry. However, the intolerance to butanol hampers the efficient production of solvents. Butanol toxicity has been attributed to the chaotropic effect on the cell
Families of serine peptidases.
N D Rawlings et al.
Methods in enzymology, 244, 19-61 (1994-01-01)
Cristina C Santini et al.
Scientific reports, 9(1), 10865-10865 (2019-07-28)
Small molecule inhibitors of BRAF and MEK have proven effective at inhibiting tumor growth in melanoma patients, however this efficacy is limited due to the almost universal development of drug resistance. To provide advanced insight into the signaling responses that
Daniela M Remus et al.
Journal of bacteriology, 195(3), 502-509 (2012-11-24)
Sortases are transpeptidases that couple surface proteins to the peptidoglycan of Gram-positive bacteria, and several sortase-dependent proteins (SDPs) have been demonstrated to be crucial for the interactions of pathogenic and nonpathogenic bacteria with their hosts. Here, we studied the role
Hui Zheng et al.
Molecules (Basel, Switzerland), 18(11), 13425-13433 (2013-11-02)
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT)
Artículos
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Protocolos
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
-
What can I use to solubilize this Product T6567, Trypsin from porcine pancreas?
1 answer-
Helpful?
-
-
How can we digest protein using Product T6567, Trypsin from porcine pancreas?
1 answer-
As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Is Product T6567, Trypsin from porcine pancreas, TPCK treated?
1 answer-
The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.
Helpful?
-
-
What is the package size of Product T6567, Trypsin from porcine pancreas?
1 answer-
Each vial content 20 ug of the product.
Helpful?
-
-
How can we get Trypsin sequence information for Product T6567, Trypsin from porcine pancreas?
1 answer-
If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
