11699121001

PCR Buffer Set

suitable for PCR, pkg of 2 × 2 mL

• UNSPSC Code: 41116133

Propiedades

• packaging: pkg of 2 × 2 mL
• manufacturer/tradename: Roche
• technique(s): PCR: suitable
• storage temp.: −20°C

Descripción

General description

The kit-provided PCR buffer without MgCl₂, in combination with the kit-provided MgCl₂ stock solution, are used for the individual adjustment of the Mg²⁺ concentration. In most PCR applications, a concentration of 1.5 mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg²⁺ concentration for high PCR specificity and amplicon yield.

Application

For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase.

Packaging

1 set containing 2 components

1 set containing 3 components

Quality

Function test: Each lot of buffer is tested in PCR.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Solo componentes del kit

• MgCl₂ Stock Solution 25 mM
• PCR Buffer without Mg²⁺ 10x concentrated
• Alkaline Phosphatase Substrate, CSPD
• Enhancer, Emerald II
• Assay Buffer

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash