11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Sinónimos: buffer
• Código UNSPSC: 41105600

Propiedades

• Formulario: solution
• Nivel de calidad: 100
• envase: pkg of 3 × 1 mL
• fabricante / nombre comercial: Roche
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Aplicación

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).[1][2]

Forma física

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Otras notas

For general laboratory use.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash