Activation is not required for this preparation. However, the beads should be washed thoroughly prior to use y with 5-10 column volumes of buffer, to remove the sodium azide in the storage buffer. Please see the link below to review the datasheet for product S1638, this may be applied to this material.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/273/s1638pis-ms.pdf
85881
Streptavidin, immobilized on Agarose CL-4B
suitable for affinity chromatography
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1 ML
$204.00
5 ML
$581.70
About This Item
UNSPSC Code:
12190000
NACRES:
NA.21
Recommended Products
form
suspension
Quality Level
contains
0.02% NaN3 as preservative
technique(s)
affinity chromatography: suitable
capacity
≥15 μg/mL, packed gel capacity (biotin)
storage temp.
2-8°C
General description
streptavidin attached to 4% beaded cross-linked agarose via a C6-spacer (1.2 mg streptavidin/ml packed gel)
Physical form
50% suspension in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.15 M sodium chloride
Other Notes
Isolation of cell surface glycoprotein by biotinylated concanavalin A and immobilized streptavidin[1].
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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I want to know if before using the beads I need activate it with any specific solution. Could you help me please.
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