A9207

Avidin−Agarose from egg white

aqueous glycerol suspension

• Synonym(s): Avidin agarose
• MDL number: MFCD00130572
• UNSPSC Code: 41106514
• NACRES: NA.56

Properties

• biological source: egg white
• conjugate: agarose conjugate
• form: aqueous glycerol suspension
• matrix: cross-linked 6% beaded agarose
• matrix activation: epichlorohydrin
• matrix attachment: amino
• matrix spacer: 15 atoms
• storage temp.: −20°C

Description

General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.

Avidin-Agarose from egg white has been used:

• in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
• in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
• in avidin bead assay for purification of labelled biotin
• for capture of glycoproteins from murine cornea samples

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable